Biolegend, 603901, Purified anti-NF-κB p105/p50 Antibody, 25μg

NF-κB p105/p50 is the class 1 member of the nuclear factor of kappa light polypeptide gene enhancer in B-cells (NF-κB) transcription factor family. NFKB1 is synthesized as a longer precursor protein, p105, which can be prolytically processed into shorter proteins, p50 and IκBγ. Both p105 and IκBγ proteins contain IκB-like ankyrin repeats that can act as NF-κB inhibitors. P50 protein forms active DNA-binding heterodimers with RelA/NF-κB p65 and c-REL. Stimulation by inflammatory signals, such as TNFα, IL-1, and LPS, lead to the proteosomal degradation of IκBα and result in the translocation of RelA/NF-κB p65:p50 and c-Rel:p50 heterodimers to the nucleus. Subsequently, the nuclear NF-κB subunits bind to target genes and regulate gene transcription. NFKB1 is a key component of the NF-κB signaling pathway and is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, and immune responses.
25μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse NF-κB p105/p50 recombinant protein (232-431 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1:5000 diluted (0.1 – 1 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone W16200A detects both p105 precursor protein and p50 subunit. As per the Human Protein Atlas RNA level dataset, Molt-4 cell is expected to have a low level of NF-?B p105/p50 expression. This clone does not work for IF microscopy application (in-house tested). This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2716155 (BioLegend Cat. No. 603901) AB_2716156 (BioLegend Cat. No. 603902)
Structure: 968 amino acids with a predicted molecular weight of 105 kD. Contains an N-terminal RHD domain, 7 ankyrin repeats, and a C-terminal death domain. Can be processed to a 50kD p50 protein and a 70kD IκBγ protein.
Distribution: Cytoplasm. Translocates to the nucleus when activated.
Function: NFKB1 belongs to the NF-κB transcription factor family and participates in the regulation of cell proliferation, differentiation, apoptosis, and immune responses.
Interaction: NFKB1 p50 forms heterodimers with NF-κB p65 and c-Rel. Interacts with MAP3K8, DSIPI, SPAG9, UNC5CL, CFLAR, NFKBIE, NFKBIZ, NFKBID, MEN1, and HIF1AN.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Lougaris V, et al. 2017. Clin Immunol. 175:99. 2. Cartwright T, et al. 2016. FEBS J. 283:1812. 3. Li SK, et al. 2015. Mol Cell Biol. 35:1619. 4. Voce DJ, et al. 2015. Oncogene. 34:2807. 5. Chang PY, et al. 2006. Cancer Res. 66:6008. 6. Coleman TA, et al. 1993. Mol Cell Biol. 13:3850.
Gene ID: 4790
UniProt: View information about NF-kappaB p105/p50 on UniProt.org
Clone: W16200A
Regulatory Status: RUO
Other Names: Nuclear factor NF-kappa-B p105/p50 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Isotype: Rat IgG2a, κ