Biolegend, 604751, Purified anti-ATP1A1 Antibody, 25μg

ATP1A1 is part of the P-type cation transport ATPases. It is the α 1 subunit of Na+/K+ ATPase which catalyzes the hydrolysis of ATP and the exchange of Na+ and K+ maintaining their balance across the plasma membrane. In addition to regulating membrane homeostasis, the α subunit (ATP1A1) particularly plays a role in mediating viral infection, e.g., coronavirus and respiratory syncytial virus. Mutations in this gene has been associated with Charcot-Marie-Tooth disease type 2DD (CMT2DD), and Hypomagnesemia, Seizures, and Mental Retardation 2 (HOMGSMR2).
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide conjugated to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 0.5 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For ICC, we recommend 4% PFA fixation followed by permeabilization with ice cold methanol, or fixation/permeabilization with ice cold methanol alone. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X.
RRID: AB_2924609 (BioLegend Cat. No. 604751) AB_2924609 (BioLegend Cat. No. 604752)
Structure: ATP1A1 can be processed by alternative splicing into 4 different isoforms; isoform 1 is a 1023 aa protein with a predicted molecular weight of 113kD.
Distribution: ATP1A1 is widely expressed in the plasma membrane of most tissues.
Function: ATP1A1 is an ATPase involved in maintaining the balance of Na+ and K+ across of the plasma membrane.
Biology Area: Cell Biology, COVID-19
Molecular Family: Enzymes and Regulators, Ion Channels
Antigen References: Takase S, et al. 2017. Sci Rep. 7:2002. Wu IC, et al. Oncotarget. 2016 Dec 20;7(51):85244-85258 Schlingmann KP, et al. 2018. Am J Hum Genet. 103:808-816. Zacherl S, et al. 2015. J Biol Chem. 290:3654-65. Azizan EA, et al. 2013. Nat Genet. 45:1055-60.
Gene ID: 476
UniProt: View information about ATP1A1 on UniProt.org
Clone: A19182B
Regulatory Status: RUO
Other Names: Sodium/potassium-transporting ATPase subunit alpha-1 (ATP1A1), Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATPase Na+/K+ transporting subunit alpha 1
Isotype: Mouse IgG2b, κ