Biolegend, 604952, Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody, 200microl

ERK1/2 (extracellular signal-regulated protein kinase) are members of the mitogen-activated protein kinase (MAPK) family of serine/threonine kinases. ERK1, also called Mitogen-activated protein kinase 3 (MAPK3) and ERK2, also called Mitogen-activated protein kinase 1 (MAPK1), are both integral effectors of the Ras-dependent extracellular signal-regulated kinase 1 (ERK1/2) mitogen-activated (MAP) kinase pathway. As such, they are vital regulators of cell cycle, differentiation, proliferation, stress-response, survival, and senescence. ERK1/2 are activated via phosphorylation of their tyrosine (Tyr204) and threonine (Thr202) residues by MEK1 and MEK2 in response to mitogenic stimuli. Upon activation, ERK1/2 translocate to the nucleus where they function in the regulation of transcription and translation. Dysregulation of ERK1/2 and the hyper-activation of the ERK pathway are characteristics of many cancers where it contributes to tumorigenesis, metastasis, and treatment-resistance, making ERK1/2 an important therapeutic target.
200microl
Verified Reactivity: Human, Mouse
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Synthetic peptide of human Erk1/2 phosphorylated at Thr202/Tyr204
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Use 20 μL per 10 mL antibody dilution buffer for each gel.
Application Notes: This clone was tested by ICC in HeLa cells treated with or without 100 ng/mL PMA. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. All three methods resulted in weak staining and a lack of phospho-specificity.
RRID: AB_2927813 (BioLegend Cat. No. 604951) AB_2927813 (BioLegend Cat. No. 604952)
Structure: ERK1 is a 379 amino acid protein with a predicted molecular weight of 43 kD. ERK2 is a 360 amino acid protein with a predicted molecular weight of 41 kD. They share 85% homology.
Distribution: Ubiquitously expressed/ Localized to the cell membrane and nucleus
Function: Serine/threonine-protein kinase, phosphatase
Interaction: Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Busca R, et al. 2016. Front. Cell Dev. Biol. 4:53. Meloche S and Pouyssegur J. 2007. Oncogene. 26:3227. Wortzel I and Seger R. 2011. Genes and Cancer. 3:195.
Gene ID: 55945595
UniProt: View information about ERK1/2 on UniProt.org
Clone: Poly21A07
Regulatory Status: RUO
Other Names: Extracellular signal-regulated kinase 1/2, Mitogen-activated protein kinase MAPK3/MAPK1, p44ERK1/p42ERK2, p44-MAPK/p42MAPK, PRKM3/PRKM2, ERK
Isotype: Rabbit Polyclonal IgG