Biolegend, 606102, Purified anti-Cytokeratin 6 Antibody, 100μg

Keratins are main structural proteins of epithelial cells and form a cytoplasmic intermediate filament network, which is required for cell integrity and protecting cells from stress or injury. Keratin 6 (KRT6), also known as cytokeratin-6 or CK-6, is a type II intermediate filament protein constitutively expressed in all major types of epithelial appendages, such as hair, nail, and glands. Expression of KRT6 is induced by cell injury, infection, and disease. KRT6 has been identified as a marker of mammary gland progenitor cells. Defects in KRT6 genes have been associated with the inherited disorder pachyonychia congenital (PC), leading to structural abnormalities of nail, palms, and soles.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human cytokeratin 6A peptide (2-18 a.a.) conjugated to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/ml (1:500-1:5000 dilution). For immunocytochemistry, a concentration range of 0.1 - 1.0 µg/ml (1:500-1:5000 dilution) is recommended. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunohistochemistry, the suggested antigen retrieval buffer is either Tris-EDTA or sodium citrate (Cat. No. 420901).
Product Citations: Koliakou E, et al. 2022. Int J Mol Sci. 23: . PubMed
RRID: AB_2734500 (BioLegend Cat. No. 606101) AB_2734501 (BioLegend Cat. No. 606102)
Structure: 564 amino acids with a predicted molecular weight of 60 kD. Type II intermediate filament.
Distribution: Cytoplasm
Function: KRT6 is a type II intermediate filament protein required for cell integrity and protecting cells from stress or injury.
Interaction: KRT6 interacts with KRT16, KRT17, and TCHP.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: Arumugam A, et al. 2014. Biochem. Biophys. Res. Commun. 451:394. Cao L, et al. 2010. J. Appl. Toxicol. 30:416. Gu LH, Coulombe PA. 2008. Am. J. Pathol. 173:752. Wong P, Coulombe PA. 2003. J. Cell Biol. 163:327. Lin MT, et al. 1999. Exp. Dermatol. 8:115. Takahashi K, et al. 1995. J. Biol. Chem. 4:18581.
Gene ID: 3853
UniProt: View information about Cytokeratin 6 on UniProt.org
Clone: W17006B
Regulatory Status: RUO
Other Names: Keratin, type II cytoskeletal 6, Cytokeratin-6, CK-6, Keratin-6, KRT6
Isotype: Rat IgG2a, κ