Biolegend, 607753, Alexa Fluor® 488 anti-Oligodendrocyte Marker O1 Antibody, 25μg

Oligodendrocytes are the myelinating cells of the central nervous system (CNS). Similar to the Schwann cells in the peripheral nervous system (PNS), their function is to provide support and isolation to neuronal axons. Their importance has been denoted on de-myelinating diseases such as multiple sclerosis, amyotrophic lateral sclerosis, periventricular leukomalacia, and also, they have been associated with dementia, schizophrenia, cognitive impairment, and bipolar disorder. Oligodendrocytes develop during embryogenesis; however, a small proportion of progenitor stem cells are present in the adult brain. Thus, three stages of differentiation can be identified in the cerebral white matter, and specific markers have been associated which each particular stage. Galactocerebroside O1 (marker O1) is one of the major galactosphingolipids components of oligodendrocytes plasma membrane. It is produced from late immature non-myelinating oligodendrocytes to mature myelinating oligodendrocytes, and together with expression of CNPase, it has been widely used as characteristic marker for these specific differentiation stages.
25μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: White matter from bovine corpus callosum (from developer)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on testis samples, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunohistochemistry on cerebellum samples, a concentration range of 0.5 - 1.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone O1 recognize specifically O1 antigen by immunocytochemistry in oligodendrocytes derived from cortical stem cell using different fixation/permeabilization methods such as methanol, PFA/Triton X-100, and PFA/methanol, where the last method shows the highest intensity.By immunohistochemistry in paraffin sections, clone O1 showed specific high staining on cerebellum white matter, and not staining in the molecular layer at the recommended concentration.By flow cytometry staining on differentiated cortical stem cells, clone O1 stained at high intensity complex cells (oligodendrocytes) compared with low complexity cells (undifferentiated progenitor stem cells).
RRID: AB_3068295 (BioLegend Cat. No. 607753) AB_3068295 (BioLegend Cat. No. 607754)
Structure: It is a galactocerebroside (glycosphingolipid), composed of a single galactose linked to a ceramide
Distribution: Present in the plasma membrane of late immature to mature oligodendrocytes.
Cell Type: Neural Stem Cells, Oligodendrocytes
Biology Area: Neurodegeneration, Neuroscience, Neuroscience Cell Markers
Antigen References: Bansal R, et al. 1989. J Neurosci Res. 24:548-57. Bansal R, et al. 1999. J Neurosci. 19:7913-24. Ahmad I, et al. 2021. PloS One. 16:e0256155. Rasti BF, et al. 2020. Int J Nanomedicine. 15:3903-3920.
Gene ID: NA
UniProt: View information about Oligodendrocyte Marker O1 on UniProt.org
Clone: O1
Regulatory Status: RUO
Other Names: Galactocerebroside O1, Oligodendrocyte marker O1, Oligodendrocyte O1 antigen
Isotype: Mouse IgM, κ