Biolegend, 608352, Purified anti-BLNK Phospho (Tyr96) Antibody, 100μg

B cell Linker protein (BLNK) is a cytoplasmic adaptor protein that is involved in B cell antigen receptor (BCR) signaling, a mechanism that is essential for the development of B lymphocytes in the bone marrow and periphery. Following BCR activation, BLNK undergoes tyrosine phosphorylation by Syk which induces interaction with effector molecules such as Grb2, Vav, and PLCγ to facilitate downstream signaling pathways. Phosphorylation sites include Tyr72, Tyr84, Tyr96, Tyr178, Tyr189, and Tyr394. Phosphorylation at Tyr96 residue facilitates BTK binding and plays a critical role in B cell maturation. BLNK mutations can block pro- to pre-B cell transitioning and result in gammaglobulinemia and absent B cells.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human BLNK phosphorylated at Tyrosine 96
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product has high background in WB if used at concentrations above 0.2 μg/mL. This product is not suitable for ICC if cells are fixed with methanol only.
RRID: AB_2936688 (BioLegend Cat. No. 608351) AB_2936688 (BioLegend Cat. No. 608352)
Structure: BLNK is a 456 amino-acid protein with a predicted molecular weight of 50 kD.
Distribution: BLNK is expressed in cytoplasm of B cells.
Function: Activation of BLNK plays a critical role in B cell proliferation, differentiation, and apoptosis.
Interaction: BLNK interacts with Grb2, Vav, PLCγ2, and Nck1.
Cell Type: B cells
Biology Area: Cell Biology
Molecular Family: Adaptor Proteins, Phospho-Proteins
Antigen References: Fu C, et al. 1998. Immunity. 9:93-103. Kurosaki T & Tsukada S. 2000. Immunity. 12:1-5. Gauld SB, et al. 2002. Science. 296:1641-2. Minegishi Y, et al. 1999. Science. 286:1954-7.
Gene ID: 29760
UniProt: View information about BLNK Phospho Tyr96 on UniProt.org
Clone: A19010A
Regulatory Status: RUO
Other Names: B Cell Linker (BLNK), B-Cell Adapter Containing ASH2 Domain Protein, SLP65, BASH
Isotype: Mouse IgG1, κ