Biolegend, 609901, Purified anti-RPS6KB1 Antibody, 25μg

Ribosomal protein S6 kinase beta-1 (RPS6KB1), also known as p70S6K, is a serine/threonine kinase participating in the PI3K/mTOR signaling pathway. Upon activation, RPS6KB1 phosphorylates ribosomal protein S6, resulting in selective translation of the 5’-terminal oligopyrimidine tract mRNAs encoding ribosomal proteins and elongation factors. RPS6KB1 also regulates translation initiation and elongation by phosphorylating eukaryotic translation initiation factor 4B (eIF4B) and eukaryotic elongation factor 2 kinase (EEF2K). RPS6KB1 has been found to be crucial in cell growth, cell cycle progression, and cell survival. Inhibition of RPS6KB1 activity results in G1 arrest and delay of entering S phase. Phosphorylation of Bcl-2-associated death promoter (BAD) by RPS6KB1 rescues cells from apoptosis. Impaired regulation of RPS6KB1 has been linked to a variety of diseases, including cancer, diabetes, and obesity.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human RPS6KB1 peptide (508-525 a.a.) conjugated to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg per ml (1:500 - 1:4000 dilution). For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/ml (1:500 - 1:1000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg for 400 µg total. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: As per the Human Protein Atlas RNA level dataset, HeLa and HEK293 cells are expected to have a low level of RPS6K1 expression. This clone recognizes two isoforms (56 and 59kD) of RPS6K1 by WB analysis.
RRID: AB_2749938 (BioLegend Cat. No. 609901) AB_2749939 (BioLegend Cat. No. 609902)
Structure: 525 amino acids with a predicted molecular weight of 59 kD. Contains a serine/threonine kinase domain.
Distribution: Nucleus. Mitochondrion.
Function: RPS6KB1 is a serine/threonine kinase mediating in the PI3K/mTOR signaing pathway. It participates in the regulation of cell growth, cell cycle progression, and cell survival.
Interaction: Interacts with PPP1R9A, RPTOR, IRS1, EIF3B, EIF3C, TRAF4, POLDIP3, and IER5.
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Chen B, et al. 2017. PLoS One. 12:e0182891. Cai C, et al. 2015. Clin Cancer Res. 21:4922. Bahrami-B F, et al. 2014. J Clin Pathol. 67:1019. Tao T, et al. 2014. Tumour Biol. 35:8543. Li PD, et al. 2012. Med Oncol. 29:3070.
Gene ID: 6198
UniProt: View information about RPS6KB1 on UniProt.org
Clone: W17145A
Regulatory Status: RUO
Other Names: Ribosomal protein S6 kinase beta-1, S6K1, 70 kD ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A (STK14A), p70 ribosomal S6 kinase alpha (p70 S6KA)
Isotype: Rat IgG2b, κ