Biolegend, 610401, Purified anti-SMAD1 Phospho (S463/S465)/SMAD9 (S465/S467) Antibody, 25μg

SMAD1 and SMAD9 (SMAD8) are members of SMAD family, whose members play a critical role in signal transduction from the TGF-β receptor kinases. Activation of these receptors results in phosphorylation of SMAD1 and SMAD9 at a C-terminal SSXS motif (Ser463/Ser465 in SMAD1; Ser465/Ser467 in SMAD9), leading to dimerization with the coactivator SMAD4. The SMAD1-4 complex is translocated to the nucleus where it induces expression of target genes involved in differentiation, self-renewal, proliferation, and apoptosis.
25μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Because the sequence of the synthetic phosphopeptide immunogen is completely conserved between SMAD1 and the corresponding residues in SMAD9 (Ser465/Ser467), this clone is predicted to recognize both phosphorylated proteins. This clone is predicted to recognize rat SMAD1 Phospho (Ser463/Ser465) and SMAD9 Phospho (Ser465/Ser467) due to complete sequence homology between the immunizing peptide and each rat homolog.
RRID: AB_2801107 (BioLegend Cat. No. 610401) AB_2801108 (BioLegend Cat. No. 610402)
Structure: SMAD1 is a 465 amino acid protein with a predicted molecular weight of 52 kD. SMAD9 is 467 amino acid protein with a predicted molecular weight of 52 kD.
Distribution: Ubiquitous expression/cytosolic and nuclear distribution
Function: Transcriptional activation/TGF- β signaling
Interaction: SMAD4, SMURF1, USP15, CBP, p300
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability, Signal Transduction, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: Hoodless PA, et al. 1996. Cell. 85: 489-500. Whitman M. 1998. Genes Dev. 12: 2445-62. Alarcon C, et al. 2009. Cell. 139: 757-69.
Gene ID: 40864093
UniProt: View information about SMAD1 Phospho Ser463/Ser465 SMAD9 Phospho Ser465/Ser467 on UniProt.org
Clone: A17019A
Regulatory Status: RUO
Other Names: Mothers against decapentaplegic homolog 1, JV4-1, Mad-related protein 1
Isotype: Mouse IgG1, λ