Biolegend, 610552, Purified anti-HOMER1 Antibody, 100μg

HOMER1 is a scaffolding protein that is a major component of the post-synaptic density (PSD), a region in the plasma membrane of the post-synaptic neuron that interacts with the pre-synaptic active zone of upstream neurons. HOMER1 consists of two domains, a C-terminal coiled-coil domain that is required for HOMER1 dimerization, and an EVH1 domain involved in protein-protein interaction. Dimerized long-form HOMER1 (variants designated as Homer1b and Homer1c) is constitutively expressed in neurons, where it plays a role in neurotransmitter receptor signaling and trafficking by cross-linking associated proteins via the EVH1 domain. Truncated HOMER1 (Homer1a) is expressed as a result of neuronal activity, and lacking the coiled-coil domain, is incapable of dimerizing and cannot crosslink interacting proteins. Thus, Homer1a acts as a dominant-negative regulator of HOMER1 and is thought to provide negative feedback against excessive neuronal activity.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human HOMER1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product is not recommended for use in Immunohistochemistry. This product does not recognize mouse or human HOMER1 in Western Blot. This product only recognizes full length HOMER1 (Homer1b/c) and not the truncated variant Homer1a.
RRID: AB_2936737 (BioLegend Cat. No. 610551) AB_2936737 (BioLegend Cat. No. 610552)
Structure: HOMER1 is a 354 amino acid protein with a predicted molecular weight of 40 kD.
Distribution: Post-synaptic density
Function: Protein-protein interactions, synaptic plasticity
Interaction: Homodimer; also interacts with other major components of the PSD including Shank family proteins, cytoskeletal proteins like dynamin, and receptors such as group 1 metabotropic glutamate receptors and the ryanodine receptor.
Cell Type: Mature Neurons, Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Synaptic Biology
Molecular Family: Postsynaptic proteins, Scaffold Proteins
Antigen References: Foa L, et al. 2001. Nat Neurosci. 4:499-506. de Bartolomeis A, et al. 2022. Neurosci Biobehav Rev. 136:104596. Salanova M, et al. 2013. IUBMB Life. 65:769-76. Reshetnikov VV, et al. 2021. Biochemistry (Mosc). 86:613-626.
Gene ID: 9456
UniProt: View information about HOMER1 on UniProt.org
Clone: A18192B
Regulatory Status: RUO
Other Names: Homer protein homolog 1 (HOMER1), SYN47
Isotype: Mouse IgG2b, κ