Biolegend, 611002, Purified anti-HDAC1 Phospho (Ser406) Antibody, 100μg

Histone Deacetylase 1 (HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and differentiation. The enzyme functions by removing acetyl moieties from histone targets, resulting in histone compaction and alterations in nucleosomal positioning.  Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. This modification plays an essential role in regulating cell cycle progression, as well as controlling the expression of genes involved in central nervous system development.
100μg
Verified Reactivity: Human, Zebrafish
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site. This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062. Clone BT-15 cross-reacts to Zebrafish2
Application References(PubMed link indicates BioLegend citation): Segre CV, et al, 2016. mAbs. 8: 37-42 Loponte S, et al, 2016. Sci Rep, 6: 30213.
RRID: AB_2801111 (BioLegend Cat. No. 611001) AB_2801112 (BioLegend Cat. No. 611002)
Structure: HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
Distribution: Nucleus/Ubiquitously expressed
Function: Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: Segre CV, et al, 2016. mAbs. 8: 37-42. Loponte S, et al, 2016. Sci Rep, 6: 30213.
Gene ID: 3065
UniProt: View information about HDAC1 Phospho Ser406 on UniProt.org
Clone: BT-15
Regulatory Status: RUO
Other Names: Histone Deactylase 1, RPD3L1
Isotype: Mouse IgG2b, κ