Biolegend, 613152, Purified anti-human HGF Antibody, 100μg

HGF is a dimeric molecule composed of the α-subunit and β-subunit, linked by a disulfide bond. The C-terminus of the α-subunit is followed directly by the N-terminus of the β-subunit. HGF protects the epithelium, neurons and cardiomyocytes during organ diseases. This protective effect is mediated through anti-apoptotic signals via inhibition of caspase-3 activity or induction of anti-apoptotic molecules, such as Bcl-xL. In addition, HGF prohibits Fas-mediated apoptosis signals via sequestration of Fas and Met receptor on cell surfaces. HGF also elicits the regression of fibrosis in numerous organs, such as scleroderma, cardiomyopathy, vocal scarring, and peritoneal fibrosis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human Recombinant HGF Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19371A) could function as a detection antibody when paired with purified anti-human HGF antibody (Cat. No. 613052) (clone W19372J) as the capture antibody and using Recombinant Human HGF protein (Cat. Nos. 596401 and 596402) as the assay standard. For assay development flexibility purpose this product is provided as purified format.
RRID: AB_3097163 (BioLegend Cat. No. 613152)
Structure: Heterodimer (alpha and beta chains)
Distribution: Mesenchymal cells, liver sinusoidal endothelial, macrophages
Function: Stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis. Hypoxia and hyperglycemia suppress HGF production. TGFβ and angiotensin-II also depress HGF production in vitro.
Interaction: Hepathocytes, epithelial cells
Ligand/Receptor: MET receptor
Cell Type: Embryonic Stem Cells
Biology Area: Angiogenesis, Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Kataoka H, et al. 2000. J Biol Chem. 275:40453-62. Wang X, et al. 2002. Mol Cell. 9:411-21. Wu MH, et al. 2004. Gene Ther. 11:170-80. Mizuno S, et al. 2008. Front Biosci. 13:7072-86. D'Angelo F, et al. 2013. Clin Exp Immunol. 174:60-72.
Gene ID: 3082
UniProt: View information about HGF on UniProt.org
Clone: W19371A
Regulatory Status: RUO
Other Names: Scatter Factor (SF), Hepatopoietin (HPTA), Lung fibroblast-derived mitogen
Isotype: Rat IgG2a, κ