Biolegend, 613252, Purified anti-human CCL1 (I-309) Antibody, 100μg

CCL1 was initially identified as a secreted protein derived from activated T cells. The CCL1/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation. As a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and a higher concentration of CCL1 in bronchoalveolar lavage of asthmatic patients compared with normal controls. In addition, high levels of CCL1 have been detected in the serum of patients with atopic dermatitis. CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1/CCR8 play a role in skin immunosurveillance. In addition, a more recent publication showed that CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β in vitro , and this transcriptional upregulation is reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Antibodies against CCL1 inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Recombinant CCL1 Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A16001E) could function as a capture antibody when paired with Biotin anti-human CCL1 antibody (Cat. No. 612253) (clone A16001C) as the detection antibody and using Recombinant Human CCL1 (I-309) (carrier-free) protein (Cat. Nos. 582702, 582704, 582706 and 582708) as the assay standard.
RRID: AB_2941611 (BioLegend Cat. No. 613252)
Structure: Chemokine
Distribution: T cells, Th1, Th2, diabetogenic T cells, peritoneal macrophages, peritoneal mesothelial cells, Langerhans cells, monocytes, mast cells, NK cells, endothelial cells, and epithelial cells
Function: CCL1 chemoattracts CD4 Th2 cells and plays a key role in the progression of Th2 type hypersensitivity.
Interaction: T cells, Th2 cells, macrophages, microglia, FOXP3+ CD4 regulatory T cells, skin-homing CLA+T cells, monocytes, NK cells, dendritic cells, endothelial cells, and smooth muscle cells
Ligand/Receptor: CCR8 (CDw198)
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Miller MD, et al. 1989. J Immunol. 143:2907-16. Haque NS, et al. 2004. Blood. 103:1296-304. Gombert M, et al. 2005. J Immunol. 174:5082-91. Montes-Vizuet R, et al. 2006. Eur Respir J. 28:59-67. Soler D, et al. 2006. J Immunol. 177:6940-51. Gonzalo JA, et al. 2007. J Immunol. 179:1740-50. Mutalithas K, et al. 2010. Clin Exp Allergy. 40:1175-85.
Gene ID: 6346
UniProt: View information about CCL1 on UniProt.org
Clone: A16001E
Regulatory Status: RUO
Other Names: T lymphocyte-secreted protein I-309
Isotype: Mouse IgG1, κ