Biolegend, 613852, Purified anti-CDC2 Phospho (Tyr15) Antibody, 100μg

Cell division control protein 2 (CDC2) also known as Cyclin-dependent kinase 1 (CDK1) is a key regulatory kinase essential for cell growth, proliferation, cell cycle progression, and early embryonic development. It is responsible for driving cells through G2 phase into M phase. Prior to mitosis, CDC2, along with its binding partner cyclin B1, exists in an inactive state when phosphorylated on Thr14 and Tyr15 by PKMYT1 (MYT1) and WEE1. This B1-CDC2 complex is activated during G2 and early mitosis by CDC2-mediated dephosphorylation. During mitosis, CDC2 inhibits DNA re-replication and subsequent polyploidy and cell senescence through the sequestering of cyclin A2 and suppression of the formation of CDK2-cyclin A complexes. CDC2 can function as a substitute for other CDC family members and drive the progression of the cell cycle in the event of their loss. CDC2 functions as a positive regulator of global translation, protein synthesis, and genome stability and participates in DNA damage repair, checkpoint activation, and DNA replication fork progression. It is overexpressed in several cancers including liver, breast, and colorectal cancers and has been implicated in the promotion of tumorigenesis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human CDC2 phosphorylated at tyrosine 15
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2941613 (BioLegend Cat. No. 613851) AB_2941613 (BioLegend Cat. No. 613852)
Structure: CDC2 is a 297 amino acid protein with a predicted molecular weight of 34 kD.
Distribution: Ubiquitously expressed/ Mitochondria, nucleus, cytoskeleton, cytoplasm
Function: Serine/threonine-protein kinase/ Cell Cycle
Biology Area: Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication
Molecular Family: Phospho-Proteins
Antigen References: Chow J, et al. 2010. Mol Cell Biol. 31:1478-91. Diril M, et al. 2012. PNAS. 109:3826-31. Haneke K, et al. 2020. J Cell Biol. 219:e201906147. Liao H, et al. 2017. Oncotarget. 8:90662-90673. Prevo R, et al. 2018. Cell Cycle. 17:1513-1523.
Gene ID: 983
UniProt: View information about CDC2 on UniProt.org
Clone: A21009D
Regulatory Status: RUO
Other Names: Cyclin Dependent Kinase 1, Cdk1, CDC28A, P34CDC2, P34 Protein Kinase
Isotype: Mouse IgG1, κ