Biolegend, 614855, Alexa Fluor® 647 anti-α Smooth Muscle Actin Antibody, 25μg

Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins expressed in mammals. α-smooth muscle actin is widely expressed in contractile cells, most notably the mural cells that line arteries and arterioles. Overexpression of α-smooth muscle actin is a hallmark of fibrosis, a thickening or scarring of damaged tissue.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: N-terminal synthetic peptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining using our True-Phos™ Perm Buffer, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Additional Product Notes: For use in immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend heat-induced antigen retrieval with either Tris-EDTA pH 9.0 or Citrate Buffer, 10X (Cat. No. 420902). For use in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X. Previously Covance catalog# MMS-466S. For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with True-Phos™ Perm Buffer (Cat. No. 425401).
RRID: AB_3662309 (BioLegend Cat. No. 614855) AB_3662309 (BioLegend Cat. No. 614856)
Structure: α smooth muscle actin is a 377 amino acid protein with a predicted molecular weight of 42 kD.
Distribution: Cytoplasmic, cytoskeleton
Function: Cell motility
Interaction: α smooth muscle actins bind to myosins to produce motive force
Cell Type: Mesenchymal Stem Cells, Monocytes
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microfilaments
Antigen References: Brennan PA, et al. 2000. J Oral Pathol Med. 29:279-83. Rizeq MN, et al. 1994. Hum Pathol. 25:671-7. Schmitt-Graff A, et al. 1991. Am J Pathol. 138::1233-42 Roholl PJM, et al. 1990. Hum Pathol. 21:1269-74. Alberts B, et al. 1989. Eds Molecular biology of the cell. 2:613. Skalli O, et al. 1986. J Cell Biol. 103:2787-96.
Gene ID: 59
UniProt: View information about alpha Smooth Muscle Actin on UniProt.org
Clone: 1A4
Regulatory Status: RUO
Other Names: Actin, aortic smooth muscle (αSMA), alpha 2 actin (ACTA2), alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein
Isotype: Mouse IgG2a, κ