Biolegend, 615002, Purified anti-GSK-3α/β Antibody, 100μg

GSK-3α/β is a ubiquitously expressed serine/threonine kinase that can phosphorylate a wide range of substrates. While GSK-3 was initially shown to regulate energy metabolism through phosphorylation of glycogen synthase, it has been shown that the enzyme has a wide variety of substrates in vivo and regulates signaling pathways that control cell growth, development,  apoptosis, and oxidative stress responses. Each isoform if encoded by separate alleles; the isoforms show 66% homology.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant full-length GSK-3β
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone recognizes both a and ß isoforms of GSK-3 by both WB and IP.This clone failed to stain PFA-fixed HeLa cells that were permeabilized with methanol or Triton-X. We therefore do not recommend using this clone for ICC.
RRID: AB_2814452 (BioLegend Cat. No. 615001) AB_2814453 (BioLegend Cat. No. 615002)
Structure: GSK-3α is a 483 amino acid protein with a predicted molecular weight of 51 kD; GSK-3β is a 420 amino acid protein with a predicted molecular weight of 47 kD.
Distribution: Both isoforms ubiquitously expressed GSK-3α: cytosol; GSK-3β: nucleus
Function: Cell Signaling
Antigen References: Imazu M, et al. 1984. J. Biol. Chem. 259: 1813. Cross DA, et al. 1995. Nature. 378: 785. Kazi A, et al. 2018. Nat. Commun. 9: 5154.
Gene ID: 29312932
UniProt: View information about GSK-3alpha/beta on UniProt.org
Clone: W17173C
Regulatory Status: RUO
Other Names: Glycogen Synthase Kinase, Glycogen Synthase Kinase 3 Alpha, Glycogen Synthase Kinase Beta, GSK3A, GSK3B
Isotype: Rat IgG2a, κ