Biolegend, 615701, Purified anti-TCF1 (TCF7) Antibody, 25μg

TCF1 is the first identified member of the T-cell-specific transcription factor family.  It plays an important role in T cell development and differentiation. TCF1 is inactivated by association with the transcriptional repressor TLE proteins. During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. Interaction with CTNNB1 results in transactivation of TCF1 target genes. Deletion of TCF1 causes massive apoptosis of double positive thymocytes, suggesting that TCF1 is required for thymocyte survival during T cell development. In addition to its function in the thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partical recombinant mouse TCF1 corresponding to amino acid residues 54-303
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per IP. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: W16175A displayed a moderately higher affinity for TCF1/TCF7 than BioLegend clone 7F11A10 when tested for WB. W16175A and 7F11A10 demonstrated similar performances when tested for IP. W16175A stained HeLa cells, which lack TCF1/TCF7, when tested for ICC; we therefore do not recommend using this clone for the application. W16175A recognizes both mouse and human TCF1/TCF7 by western blot; 7F11A10 is specific for human TCF1/TCF7. Both W16175A and 7F11A10 recognize TCF1/TCF7 isoforms lacking the ß-catenin binding domain.
Product Citations: Lee J, et al. 2024. Nat Commun. 7688:15. PubMed
RRID: AB_2814458 (BioLegend Cat. No. 615701) AB_2814459 (BioLegend Cat. No. 615702)
Structure: TCF1 is a 384 amino acid protein with a predicted molecular weight of 42 kD.
Distribution: Tregs/nuclear distribution
Function: TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+/CD8+ double positive thymocytes and differentiation of T cells in the periphery.
Cell Type: T cells
Biology Area: Transcription Factors
Antigen References: Germar K, et al. 2011. P.roc Natl. Acad. Sci. USA 108:20060-5. Wang R, et al. 2011. J. Immunol. 187:5964. Weber BN, et al. 2011. Nature 476:63. Jeannet G, et al. 2010. Proc. Natl. Acad. Sci. USA 107:9777-82. Staal FJ, et al. 1999. Int. Immunol. 11:317.
Gene ID: 6932
UniProt: View information about TCF1 on UniProt.org
Clone: W16175A
Regulatory Status: RUO
Other Names: Transcription Factor 7, Transcription Factor 7 (T-Cell Specific, HMG-Box), T-Cell-Specific Transcription Factor 1, T-Cell Factor-7, T-Cell Factor 1, TCF1
Isotype: Rat IgG2a, κ