Biolegend, 619153, PE anti-EphB4 Antibody, 25tests

Ephrin type-B receptor 4 (EphB4) is a member of the Eph family of receptor tyrosine kinases that bind to the Ephrin family of ligands. Specifically, EphB4 binds to Ephrin B2, in a process involving direct cell-to-cell interaction, to initiate signaling pathways that influence cell behavior such as differentiation, adhesion and migration.  EphB4 has been shown to be involved in heart morphogenesis and angiogenesis, and in the tumorigenesis of cancers such as gliomas, breast and prostate cancer.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human EphB4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This product is not recommended for use in IHC-P. This product does not recognize mouse EphB4 in WB. For ICC, we recommend fixation/permeabilization with 100% ice-cold methanol. We do not recommend using 4% PFA as a fixative.
RRID: AB_3662310 (BioLegend Cat. No. 619153) AB_3662310 (BioLegend Cat. No. 619154)
Structure: EphB4 is a 987 amino-acid protein with a predicted molecular weight of 108.3 kD.
Distribution: Plasma membrane
Function: Receptor tyrosine kinase
Ligand/Receptor: Ephrin B2
Biology Area: Angiogenesis, Cell Biology, Neuroscience, Signal Transduction
Antigen References: Piffko A, et al. 2022. Int J Mol Sci. 23:1679. Chen Y, et al. 2017. Semin. Cancer Biol. 56:37-46. Du E, et al. 2020. Mol Biol Rep. 4:4681-4690.
Gene ID: 2050
UniProt: View information about EphB4 on UniProt.org
Clone: W20295K
Regulatory Status: RUO
Other Names: Ephrin type-B receptor 4 (EphB4), HTK, MYK1, TYRO11
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.