Biolegend, 620752, Purified anti-Sun2 Antibody, 100μg

Sun2 is a transmembrane protein that localizes to the inner nuclear membrane and interacts with other nuclear proteins to form the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. This complex bridges the nuclear envelope, connecting the nuclear lamina to the cytoskeleton, thereby providing mechanical support and stability to the nucleus. Sun2 also plays a vital role in nuclear migration, chromosome organization, and gene regulation. Additionally, Sun2 is involved in maintaining nuclear envelope integrity during cell division and DNA repair, and has been suggested to act as a tumor suppressor by protecting against cancer-inducing DNA damage. In lung cancer, Sun2 has been shown to inhibit the Warburg effect via Glut1 and LDHA.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Residues 1-121 of human SUN2 fused to GST
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For IHC-P, antigen retrieval using Citrate buffer, pH 6.0 (Cat. No. 420901) is recommended. For ICC, we recommend fixation/permeabilization with 100% ice-cold methanol for 10 minutes. Other fixation/permeabilization methods have not been tested.
RRID: AB_3097218 (BioLegend Cat. No. 620751) AB_3097218 (BioLegend Cat. No. 620752)
Structure: Sun2 is a 717 amino acid protein with a predicted molecular weight of 80.3 kD.
Distribution: Nuclear membrane, ubiquitously expressed in all cell types
Function: Structural protein
Interaction: Sun1, Nesprin 1g, Nesprin 1α
Biology Area: Cell Motility/Cytoskeleton/Structure, Cell Structure
Molecular Family: Nuclear Markers
Antigen References: Stroud M, et al. 2014. Circ. Res. 114538-48. Chen X, et al. 2019. Oncol Lett. 17:01-1408. Meinke P, et al.2011. Biochem Soc Trans. 39:1693-7. Lv X, et al. 2016. Sci Rep. 5:17940.
Gene ID: 25777
UniProt: View information about Sun2 on UniProt.org
Clone: 3.1E
Regulatory Status: RUO
Other Names: Sad1 and UNC84 domain containing 2, UNC84B,SUN Domain-Containing Protein 2, Rab5-Interacting Protein, FRIGG, Protein unc-84 homolog B, Rab5IP, Sad1/unc-84 protein-like 2
Isotype: Mouse IgG1, κ