Biolegend, 621152, Purified anti-FAP Antibody, 100μg

Fibroblast activation protein (FAP) is a cell surface and soluble protein with diverse functions in tissue homeostasis and diseases. As a prolyl peptidase, FAP cleaves peptide bonds following a proline residue. Primarily expressed by activated fibroblasts, FAP plays a crucial role in tissue remodeling and repair. Capable of being shed into the cellular milieu, FAP is involved in the breakdown and modification of the extracellular matrix, which is essential for tissue regeneration during wound healing and tissue repair. Moreover, FAP has been found to be overexpressed in the tumor microenvironment, where it potentially promotes tumor growth, invasion, and metastasis. This association with cancer has led to investigations exploring FAP as a potential therapeutic target for cancer treatment. FAP is inactive when monomeric and must form a homodimer to become functional for both the cell surface and soluble forms.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human FAP
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Product is not suitable for ICC. Product does not react with mouse FAP in Western Blot. For IHC-P, antigen retrieval using sodium citrate, pH 6.0 is recommended. Tris-EDTA-based antigen retrieval solutions are not recommended.
RRID: AB_3083407 (BioLegend Cat. No. 621151) AB_3083407 (BioLegend Cat. No. 621152)
Structure: FAP is a 760 amino acid protein with a predicted molecular weight of 87.7 kD.
Distribution: Cell surface, secreted
Function: Protease
Interaction: Degrades gelatin, DPP4, integrin alpha-3/beta-1, ITGB1, PLAUR
Cell Type: Fibroblasts
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family: Proteases
Antigen References: Lee KN, et al. 2006. Blood. 107:1397-404. Waumans Y. et al. 2015. Front Immunol. 6:387. Kotackova L, et al. 2009. Folia Biol. (Praha). 55:77-84. Sulda ML, et al. 2006. Adv Exp Med Biol. 575:197. Aertgeerts K, et al. 2005. J Biol Chem. 280:19441-4. Mueller S, et al. 1999. J Biol Chem. 274:24947-52.
Gene ID: 2191
UniProt: View information about FAP on UniProt.org
Clone: A21107A
Regulatory Status: RUO
Other Names: Prolyl endopeptidase FAP, fibroblast activation protein alpha (FAP-alpha), 170 kDa melanoma membrane-bound gelatinase, dipeptidyl peptidase FAP (DPPIV), Integral membrane serine protease, Post-Proline Cleaving Enzyme, SIMP
Isotype: Rat IgG2b, κ