Biolegend, 621553, Ultra-LEAF™ Purified anti-human Artemin Antibody, 100μg

Artemin belongs to the GDNF family of ligands consisting of 4 members: GDNF, Neurturin, Artemin, and Persephin. GDNF family members orchestrate multiple aspects of the development and maintenance of the central and peripheral nervous systems. These GDNF family ligands signal through a multicomponent receptor system composed of a common signaling component RET (receptor tyrosine kinase) and one of the high affinity binding coreceptors GFRα (α1-α4). Artemin is a GDNF family member that functions as a ligand for the GFRα3 and RET complex. Artemin is a disulfide-linked homodimer that contains three intra-chain disulfide linkages and a cysteine knot motif. Artemin supports the survival of sensory and sympathetic peripheral neurons and also supports survival of dopaminergic midbrain neurons. Artemin expression in adult tissues is ubiquitous with high levels in peripheral tissues including the prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. In adult rat, systemic artemin treatment promoted the regeneration of sensory axons to the brainstem after brachial dorsal root crush. Recent findings suggest Artemin regulates multiple aspects of carcinoma progression and metastasis. Artemin is a hypoxia-responsive factor and is essential for hypoxia-induced cancer stem cell expansion in hepatocellular carcinoma and Artemin stimulates de novo tumor angiogenesis in mammary carcinoma mediated in part by VEGF-A.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human Artemin
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.2 µg/mL human GFRα3/GFRA3-Fc chimera to 1 µg/mL immobilized recombinant human Artemin. ND50 range: 0.2-1.2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3083410 (BioLegend Cat. No. 621553) AB_3083410 (BioLegend Cat. No. 621554)
Structure: Disulfide-linked homodimer
Distribution: Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis
Function: Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons
Ligand/Receptor: CGFRα3, GFRα1
Cell Type: Embryonic Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Stem Cells
Molecular Family: Adhesion Molecules, Growth Factors
Antigen References: Baloh RH, et al. 1998. Neuron. 21:1291-1302 Masure S, et al. 1999. Eur J Biochem. 266:892-902 Gardell LR, et al. 2003. Nature Med. 9:1383-1389 Honma Y, et al. 2002. Neuron. 35:267-282 Arindam B, et al. 2012. PLoS One. 7:e50098 Wong LE, et al. 2015. Proc Natl Acad Sci USA. 112:6170-5 Zhang M, et al. 2016. Oncotarget. 7:3267-82
Gene ID: 9048
UniProt: View information about Artemin on UniProt.org
Clone: A21110A
Regulatory Status: RUO
Other Names: ARTN, ART, Enovin, Neublastin, EVN, NBN
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.