Biolegend, 622354, Ultra-LEAF™ Purified anti-human TSLPR Antibody, 1mg

TSLP-R, also known as the thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind with TSLP. TSLP-R contains a WSXWS motif required for proper protein folding and a box 1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight of approximately 40 kD, and has two isoforms reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells, dendritic cells, and activated monocytes. It is weakly expressed on T cells. Expression has also been reported in heart, skeletal, muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. Ligand receptor interactions have been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in an allergic response.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human TSLPR
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the proliferation of Baf3-hTSLPR/hIL-7Ra transfectant cells induced by recombinant human TSLP at 10 ng/mL. ND50 range: 0.6 - 4 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3083411 (BioLegend Cat. No. 622353) AB_3083411 (BioLegend Cat. No. 622354)
Structure: Type I membrane receptor
Distribution: Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells; also expressed in heart, skeletal, muscle, and kidney cells.
Function: TSLP binding to functional receptor activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. It has also been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells
Interaction: Forms functional heterodimeric complex with IL-7R to bind TSLP
Ligand/Receptor: TSLP/TSLPR
Cell Type: Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Reche PA, et al. 2001. J Immunol. 167:336-43. Tonozuka Y, et al. 2001. Cytogenet. Cell. Genet. 93:23-5. Zhang W, et al. 2001. Biochem Biophys Res Commun. 281:878-83. Wang YH, et al. 2006. Immunity, 24:-838.
Gene ID: 64109
UniProt: View information about TSLPR on UniProt.org
Clone: W20296I
Regulatory Status: RUO
Other Names: Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.