Biolegend, 624051, Purified anti-γ-Catenin Antibody, 25μg

γ-Catenin, also called plakoglobin or JUP, is a member of the armadillo family of signaling molecules. γ-Catenin plays an important role in cell adhesion with α-catenin and β-catenin, participates in Wnt signaling, embryonic development and tumorigenesis. Though β-catenin and γ-Catenin share sequence homology, γ-catenin has distinct N- and C-terminal regions which imparts distinct functions through interaction with various protein partners. Recent evidence indicates that γ-Catenin modulates desmosomal adhesion in response to growth factor stimulation. Transgenic mice homozygous with a null mutation of the γ-Catenin gene die during the embryonic stage, approximately on the 12th day of development, due to significant abnormalities in the adherens junctions and the absence of properly functioning desmosomes in the heart. The alteration of the γ-Catenin gene has been suggested as a contributing factor in the development of the heart disease called arrhythmogenic right ventricular dysplasia (ARVD) or arrhythmogenic right ventricular cardiomyopathy. Specifically, the mutations in γ-Catenin result in an autosomal recessive type of the disease known as Naxos disease.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial Recombinant human γ-Catenin protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone cross-reacts with mouse γ-Catenin. For immunocytochemistry (ICC), the suggested fixation/permeabilization method is either 4% paraformaldehyde/0.5% Triton X-100 or paraformaldehyde followed by ice-cold methanol or fixed with ice-cold methanol only. For immunohistochemistry (IHC-P), the suggested antigen retrieval buffer is Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0. For ICFC (intracellular flow cytometry) staining with this clone, we recommended using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). True-Phos™ Perm Buffer (Cat. No. 425401) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) are not applicable.
RRID: AB_3097234 (BioLegend Cat. No. 624051) AB_3097234 (BioLegend Cat. No. 624052)
Structure: γ-Catenin is a 745 amino acid protein with a predicted molecular weight of 82 kD
Distribution: Cardiac cells
Function: Regulation of cell-cell adhesion
Interaction: MUC1, CAV1, PTPRJ, DSG1, DSC1, DSC2, PKP2
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Signal Transduction
Antigen References: Zhurinsky J, et al. 2000. J Cell Sci. 113:3127-39. Zhurinsky J, et al. 2000. Mol Cell Biol. 20:4238-53. Charpentier E, et al. 2000. J Cell Biol. 149:503-20. Kolligs FT, et al. 2000. Genes Dev. 14:1319-31. Yin T, et al. 2005. J Biol Chem. 280:40355-63. Ruiz P, et al. 1996. J Cell Biol. 135:215-25. Bierkamp C, et al. 1996. Dev Biol. 180:780-85. Zhang Z. et al. 2015. J Clin Invest. 125:1708-12. Li D. et al. 2015. J Biol Chem. 287:10435-43. Rampazzo A. 2006. Heart Int. 2:17.
Gene ID: 3728
UniProt: View information about gamma-Catenin on UniProt.org
Clone: W22004E
Regulatory Status: RUO
Other Names: Catenin gamma, Desmoplakin III, desmoplakin-3, DP3CTNNG, DPIII, gamma-catenin, junction plakoglobin, PDGBARVD12, PKGB, JUP, dp3, Catenin (Cadherin-associated Protein), Gamma 80kDa, CTNNG, PDGB, Desmoplakin 3
Isotype: Rat IgG2b, κ