Biolegend, 624951, Purified anti-CGN Antibody, 25μg

CGN, found in the cytoplasmic plaque of tight junctions, is situated on the cytoplasmic side of tight junctions in polarized epithelia and certain endothelia. It is made up of a homodimer structure, with each subunit having a globular "head" section, a coiled-coil "rod" section, and a tiny globular "tail" section. Furthermore, CGN binds to JAM, ZO proteins, AF-6, and myosin, indicating its potential role in transmitting the mechanical energy produced by the contraction of the actomyosin cytoskeleton to the proteins of the cytoplasmic plaque and the membrane regions of TJs. Its main function is likely in the formation and control of the tight junction paracellular permeability barrier. Cingulin has additionally been discovered to engage with microtubules via the N-terminal head area, and these connections were governed by the process of phosphorylation by the adenosine monophosphate-activated protein kinase (AMPK). Moreover, the production of CGN in initial mouse embryos is specific to certain tissues, and it takes place in the blastocyst (increased in trophectoderm and decreased in inner-cells).
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial Recombinant human CGN protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone does not cross-react with mouse. For immunocytochemistry (ICC), the suggested fixation/permeabilization method is either 4% paraformaldehyde/0.5% Triton X-100 or paraformaldehyde followed by ice-cold methanol, or fixed with ice-cold methanol only. This product is not recommended for use in immunohistochemistry.
Additional Product Notes: This antibody has been tested in knockout/knockdown models for Western Blotting and Immunocytochemistry.
RRID: AB_3097248 (BioLegend Cat. No. 624951) AB_3097248 (BioLegend Cat. No. 624952)
Structure: CGN protein is a 1203 amino acid protein with a predicted molecular weight of 137 kD
Distribution: Pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart
Function: Formation and regulation of the tight junction
Interaction: TJP1/ZO1, SPEF1
Cell Type: Endothelial cells, Epithelial cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References: Cordenonsi M, et al. 1999. J Cell Biol. 147:1569–82. Bazzoni G, et al. 2000. J Biol Chem. 275:20520-6. Yano T, et al. 2013. J Cell Biol. 203: 605–14. Javed Q, et al. 1993. Development. 117:1145-51. Fleming TP, et al. 1993. Development. 117:1135-44.
Gene ID: 57530
UniProt: View information about CGN on UniProt.org
Clone: W21116A
Regulatory Status: RUO
Other Names: Cingulin, KIAA1319
Isotype: Rat IgG2a, κ