Biolegend, 626201, Purified anti-Syk Antibody, 25μg

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites. The SYK-01 monoclonal antibody (raised against amino acids 5-360 of human Syk) recognizes human, mouse, and rat and Syk and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
25μg
Verified Reactivity: Human, Rat, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant (amino acids 5-360) human Syk
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - VerifiedIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 0.5 µg per ml. For IHC, use a 10 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation.This clone may produce a non-specific band of roughly ~250 kD at higher antibody concentrationsThis clone does not recognize ZAP70This clone has been reported to recognize rat Syk
Application References(PubMed link indicates BioLegend citation): Arudchandran R, et al. 2000. J. Exp. Med. 191:47. (IP WB) Swann PG, et al. 1999. J. Biol. Chem. 33:23068. (IP) Inoue M, et al. 2011. J. Immunol. 186:18. PubMed Hatterer E, et al. 2011. Neurosci Res. 70:172. PubMed Cunningham CC, et al. 2012. Clin Immunol. 144:228. PubMed Alsadeg A, et al. 2014. J Immunol. 193:268. PubMed
Product Citations: Duque-Afonso J, Lin CH 2016. Cancer Res . 76(23):6937-6949. PubMed Hatterer E, et al. 2011. Neurosci Res. 70:172. PubMed Cunningham C, et al. 2012. Clin Immunol. 144:228. PubMed Zheng S, et al. 2019. PLoS Biol. 17:e3000569. PubMed Hüttl S, et al. 2015. J Immunol. 195: 1548-1563. PubMed Inoue M, et al. 2011. J Immunol. 186:19:00. PubMed Alsadeq A, et al. 2014. J Immunol. 193:268. PubMed
RRID: AB_493087 (BioLegend Cat. No. 626201) AB_2200268 (BioLegend Cat. No. 626202)
Structure: Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Distribution: Cytoplasmic protein, widely expressed in hematopoietic cells
Function: Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
Interaction: c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, paxillin, FAK, Grb2, CD3ε, LAT, many others
Modification: Phosphorylation (Y131, Y323, Y348, Y352, Y525, Y526)
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790. 2. Toyabe S-L, et al. 2001.Immunology 103:164.
Gene ID: 6850
UniProt: View information about Syk on UniProt.org
Clone: SYK-01
Regulatory Status: RUO
Other Names: Spleen tyrosine kinase, protein tyrosine kinase SYK
Isotype: Mouse IgG1, κ