Biolegend, 626501, Purified anti-Fyn Antibody, 25μg

Fyn is a 59 kD protein tyrosine kinase and a member of the src subfamily. There are two reported isoforms of Fyn; FynT found in T cells, B cells and thymocytes, and FynB found in neuronal cells. Fyn has been shown to control cell growth, interact with the p85 subunit of PI3K, control high affinity IgE receptor-mediated mast cell degranulation, and participate in neuronal cell signaling. Fyn can be activated by T cell receptor engagement or anti-CD3 ligation. Fyn is known to be modified by phosphorylation and palmitoylation and has been shown to interact with Fyn binding protein, p85 PI3K, high affinity IgE receptor, and NCAM140. The FYN-59 monoclonal antibody recognizes human and mouse Fyn and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant human Fyn (aa 7-176) expressed in E coli
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store antibody solution undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Laird RM, et al. 2010. J. Immunol. 185:6518. PubMed
Product Citations: Doi H, et al. 2014. Clin Immunol. 184:150. PubMed Nelson MH, et al. 2015. J Immunol. 1737:194. PubMed Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed Shi W, et al. 2020. J Biol Chem. 295:7653. PubMed Dwyer A, et al. 2016. J Leukoc Biol. 100: 163 - 175. PubMed Laird R, et al. 2010. J Immunol. 185:6518. PubMed Laird R, Hayes S 2010. PLoS One. 5:e8899. PubMed Lautz JD, et al. 2021. Cell Rep. 37:110076. PubMed Bland T, et al. 2019. Endocrinology. 161(2):. PubMed
RRID: AB_2108785 (BioLegend Cat. No. 626501) AB_2278824 (BioLegend Cat. No. 626502)
Structure: Protein tyrosine kinase family, src subfamily. Isoforms fynT, fynB, 59 kD
Distribution: FynT T cells, thymocytes, B cells; fynB neuronal cells
Function: Controls cell growth, interacts with p85 subunit of PI3K. Involved in high affinity IgE receptor-mediated mast cell degranulation
Interaction: Fyn binding protein, p85 PI3K, high affinity IgE receptor, NCAM140
Modification: Phosphorylation, Palmitoylation
Cell Type: T cells, Thymocytes, B cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Goldsmith J, et al. 2002. Biochem. Biophys. Res. Comm. 298:501. 2. Prasad K, et al. 1993. P. Natl. Acad. Sci. USA 90:7366. 3. Kawakami T, et al. 1986. Mol. Cell. Biol. 6:4195.
Regulation: Activated by TCR engagement or anti-CD3
Gene ID: 2534
UniProt: View information about Fyn on UniProt.org
Clone: FYN-59
Regulatory Status: RUO
Other Names: Tyrosine kinase p59fyn, Protein-tyrosine kinase fyn, Proto-oncogene tyrosine-protein kinase fyn
Isotype: Mouse IgG