Biolegend, 626804, Direct-Blot™ HRP anti-c-Myc Antibody, 25microl

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.
25microl
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Amino acids 408-439, C-terminal region of human c-myc
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: *Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
Application References(PubMed link indicates BioLegend citation): Rao H, et al. 2001. Nature. 410:955. Peters CS, et al. 2001. J Biol Chem. 276:13718. Munro S, Pelham HR. 1987. Cell. 48:899. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations: Calvo IA, et al. 2021. iScience. 24:103338. PubMed
RRID: AB_2783361 (BioLegend Cat. No. 626804) AB_2572009 (BioLegend Cat. No. 626803)
Biology Area: Cell Biology, Protein Purification, Protein Synthesis
Antigen References: Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217. Battey J, et al. 1983. Cell 34:779. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID: 4609
UniProt: View information about c-Myc on UniProt.org
Clone: 9E10
Regulatory Status: RUO
Other Names: myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Isotype: Mouse IgG1, κ