Biolegend, 626902, Purified anti-cdc2 (p34) Antibody, 100μg

Cdc2 (p34), also known as cell division cycle 2, G1 to S and G2 to M; cyclin dependent kinase 1, p34 kinase, and CDK1, is serine/threonine kinase that is ubiquitously expressed in eukaryotes. Cdc2 is found as both a cytoplasmic and nuclear protein. cdc2 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis universally in eukaryotes. The cdc2 protein is highly phosphorylated on threonine, serine, and tyrosine; tyrosine phosphorylation is subject to cell cycle regulation. Cdc2 has been shown to interact with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, and p53. The POH-1 antibody recognizes human, monkey and bovine cdc2 and is useful for Western blotting. The POH-1 antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (formalin-fixed, paraffin embedded), and immunofluorescence.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human cdc2 protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: This antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified IP, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not react with Mouse, Rat, Xenopus, Drosophila. Additional reported applications (for the relevant formats) include: immunohistochemistry1 of formalin-fixed, paraffin-embeded tissue sections and immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Lukas J, et al. 1992. Eur. J. Biochem. 207:169.
Product Citations: Chilamakuri R, et al. 2022. Pharmaceuticals (Basel). 15:. PubMed Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed Liu Y, et al. 2014. J Control Release. 192:114. PubMed Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
RRID: AB_2074779 (BioLegend Cat. No. 626901)
Structure: Serine/threonine kinase; molecular weight approximately 34 kD
Distribution: Ubiquitous expression in eukaryotes, cytoplasmic and nuclear.
Function: cdc2 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis universally among eukaryotes.
Interaction: Interacts with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, p53
Modification: Highly phosphorylated (threonine, serine, tyrosine); tyrosine phosphorylation subject to cell cycle regulation
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Draetta G, et al. 1988. Nature 336:738.
Gene ID: 983
UniProt: View information about cdc2 p34 on UniProt.org
Clone: POH-1
Regulatory Status: RUO
Other Names: Cell division cycle 2, G1 to S and G2 to M, cyclin dependent kinase 1, p34 kinase, CDK1
Isotype: Mouse IgG2a, κ