Biolegend, 628606, Alexa Fluor® 594 anti-Cytokeratin (pan reactive) Antibody, 100μg

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Keratin enriched fraction from human epidermoid carcinoma.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and spatial biology (IBEX)7,8.
Application References(PubMed link indicates BioLegend citation): Kovarik J. 1988. Int. J. Cancer Suppl. 3:50. Bartek J, et al. 1991. J. Pathol. 164:215. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed Comito G, et al. 2012. Cancer Lett. 324:31. PubMed Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow) Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Sudmeier LJ, et al. 2022. Cell Rep Med. 3:100620. PubMed Sun YF, et al. 2021. Nat Commun. 12:4091. PubMed
RRID: AB_2566437 (BioLegend Cat. No. 628606)
Structure: Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits
Distribution: Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus
Function: Intermediate filament protein involved with the cytoskeleton.
Interaction: Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Gene ID: 3875
UniProt: View information about Cytokeratin pan reactive on UniProt.org
Clone: C-11
Regulatory Status: RUO
Other Names: Cytokeratin, Keratin
Isotype: Mouse IgG1, κ