Biolegend, 628852, Purified anti-VWF Antibody, 100μg

Von Willebrand factor (VWF) plays a crucial role in blood clotting by assisting platelets in forming clots at the site of blood vessel injury. Acting as a bridge between platelets and the damaged blood vessel walls, VWF facilitates platelet adhesion and aggregation, which are essential steps in the clotting process. Moreover, VWF also stabilizes factor VIII, a key protein in the blood clotting cascade, preventing its degradation and enhancing its availability for clot formation. Mutations or deficiencies in VWF results in defects in platelet adhesion and potentially bleeding disorders such as thrombotic thrombocytopenic purpura, Heyde’s syndrome, and von Willebrand disease. A critical region of VWF is the A2 domain. Contained in this domain is the cleavage site for the ADAMTS13 protease which inactivates VWF.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant portion of human VWF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone A21125D/G) is not suitable for use in western blot. This antibody (clone A21125D/G) is a pool of two monoclonal antibodies, A21125D and A21125G. For intracellular flow cytometry (ICFC), fixation/permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401) is recommended.
RRID: AB_3097540 (BioLegend Cat. No. 628851) AB_3097540 (BioLegend Cat. No. 628852)
Structure: VWF is a 2,813 amino acid protein with a predicted molecular weight of 309 kD.
Distribution: ECM, secreted, cytoplasmic
Function: Structural protein, multimeric
Interaction: Factor VIII (F8), ADAMTS13, GPIb receptor, heparin, collagen, platelet integrin αIIbβ3
Cell Type: Platelets
Antigen References: Randi AM, et al. 2018. Blood. 132:132-40. Xiang Y & Hwa J. 2016. Curr Opin Hematol. 23:288-93. Chen J & Chung DW. 2018. Blood. 132:141-147. Lynch CJ, et al. 2014. Blood. 123:2585-92.
Gene ID: 7450
UniProt: View information about VWF on UniProt.org
Clone: A21125D/G
Regulatory Status: RUO
Other Names: von Willebrand factor (vWF), F8VWD, VWD, Coagulation factor VIII antibody, Coagulation factor VIII VWF antibody
Isotype: Mouse IgG2b, κ