Biolegend, 628902, Purified anti-PLK-1 Phospho (Thr210) Antibody, 100μg

PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 2A3 antibody recognizes human phosphorylated PLK-1 (Thr210) and has been shown to be useful for Western blotting. To increase specificity, it is recommended that the 2A3 antibody be used for Western blotting after immunoprecipitation with the pan-specific PLK-1 3F8 antibody.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Modified peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Molli PR, et al. 2010. J Cell Biol. 190:101. PubMed Yang S, et al. 2013. Cancer Res. 73:6722. PubMed
Product Citations: Pop OT, et al. 2023. Cell Mol Gastroenterol Hepatol. 17:16. PubMed Bucko PJ, et al. 2019. eLife. 0.333333333333333. PubMed Bucko PJ, et al. 2020. J Biol Chem. 295:13784. PubMed Yang S, et al. 2013. Cancer Res. 73:6722. PubMed Molli P, et al. 2010. J Cell Biol. 190:101. PubMed Tankiewicz‐Kwedlo A et al. 2017. British journal of pharmacology. 175(5):743-762 . PubMed
RRID: AB_439786 (BioLegend Cat. No. 628901) AB_439787 (BioLegend Cat. No. 628902)
Structure: Serine/Threonine family of protein kinases, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD
Distribution: Nuclear protein, highly expressed in placenta and colon
Function: Regulates cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis
Interaction: Interacts with nuclear distribution gene C
Modification: Phosphorylation (Thr210 is major phosphorylation site in activated PLK-1 from mitotic cells)
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: 1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249. 2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793. 3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.
Regulation: Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2/M phase and declines to nearly undetectable levels after mitosis and throughout G1)
Gene ID: 5347
UniProt: View information about PLK-1 Phospho Thr210 on UniProt.org
Clone: 2A3
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Serine/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13
Isotype: Mouse IgG1, κ