Biolegend, 629753, Ultra-LEAF™ Purified anti-human Ephrin-A1 Antibody, 100μg

Ephrins are cell surface molecules with a wide range of biological functions that are involved in both embryogenesis and adult life. This includes important roles in boundary formation, cell migration, repulsive axon guidance, and the regulation of neuronal growth cone development. The ephrin family consists of eight members. Ephrin-A1 through A5 are anchored to the cell membrane via GPI linkage, while ephrin-B1 through B3 are transmembrane proteins. The interaction between ephrin ligands and its cognate Eph receptors involve both forward and reverse signaling. Ephrin-A1 was originally isolated in conditioned media from HUVEC cells treated with TNF-α, and the gene was found to be induced by TNF-α in these cells. Ephrin-A1 is broadly expressed on endothelial cells and epithelial cells, but it can also be released as a soluble molecule. Ephrin-A1 and its primary receptor EphA2 play a vital role in normal angiogenesis and tumor neovascularization. Ephrin-A1 expressed on tumor cells inhibits tumor cell proliferation and migration, but also supports tumor growth by promoting angiogenesis. Ephrin-A1 is also expressed on resting CD4+ T cells and its expression is down regulated in asthma patients.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Ephrin-A1
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.1 µg/mL biotinylated human Ephrin-A1-Fc chimera to 5 µg/mL immobilized recombinant human EphA1-Fc chimera. ND50 range: 0.02 - 0.14 µg/mL.
RRID: AB_3683476 (BioLegend Cat. No. 629753) AB_3683476 (BioLegend Cat. No. 629754)
Structure: Monomer and Homodimer
Distribution: Widely expressed in differentiated epithelial cells in bone marrow, spleen, thymus, and testes. Also expressed on CD4+ T cells and a subpopulation of CD19+ B cells
Function: Regulation of angiogenesis, axongenesis, endothelial cell migration, integrin mediated adhesion, notochord formation, and negative regulation of epithelial to mesenchymal transition
Interaction: Endothelial cells, epithelial cells, resting CD4+ T cells
Ligand/Receptor: EphA2
Cell Type: Endothelial cells, Macrophages, NK cells, T cells
Biology Area: Angiogenesis, Cell Biology, Immuno-Oncology, Neurodegeneration, Neuroscience
Antigen References: Lisabeth EM, et al. 2013. Cold Spring Harb Perspect Biol. USA. 5:9. Pasquale EB. 2008, Cell. 133:38-52. Hirai H, et al. 1987. Science. 238:1717-20. Aasheim HC, et al. 2005. Blood. 105:2869-76. Coulthard MG, et al. 2001. Growth Factors. 18:303-17. Ieguchi K and Maru Y. 2019. Cancer Sci. 3:841. Inokuchi M, et al. 2018. Anticancer Res. 3:1685 Jin Y, et al. 2018. Mol Med. Rep. 17:5409. Chu M and Zhang C. 2018. Sci Rep. 1:1539 Darling TK and Lamb TJ. 2019. Front Immunol. 10:01473.
Gene ID: 1942
UniProt: View information about Ephrin-A1 on UniProt.org
Clone: A16015A
Regulatory Status: RUO
Other Names: EFNA1, EPLG1, TNFAIP4, LERK1, EFL1, B61
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.