Biolegend, 630052, Purified anti-ATF-2 Antibody, 100μg

The Activating Transcription Factor 2 (ATF-2), also known as CREB2 and CREBP1, contains a Basic Leucine Zipper (bZIP) domain and a C2H2-type zinc finger domain. ATF-2 binds to both DNA response factors AP-1 and CRE and belongs to the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cytosolic tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. ATF-2 activity can be initiated by various forms of cellular stress. Several studies have shown aberrant ATF-2 activation in the development and progression of mammalian skin tumors. ATF-2 may mediate carcinogenesis induced by the mutant Ras protein and regulate the maintenance of the invasive cancer phenotype of several epithelial cell types. ATF-2 has also been shown to be phosphorylated at its C-terminus (serine 472 and 480 in mice, serine 490 and 498 in human) by ATM during double-strand breaks. Mice with mutations of these two serines are sensitive to irradiation and more prone to tumorigenesis under p53 knockout condition.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial Recombinant human ATF2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone cross-reacts with mouse in western blot (WB). For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 is recommended. This product is not recommended for use in immunohistochemistry (IHC).
Additional Product Notes: This antibody has been tested in knockout/knockdown models for Western Blotting and Immunocytochemistry.
RRID: AB_3097336 (BioLegend Cat. No. 630051) AB_3097336 (BioLegend Cat. No. 630052)
Structure: ATF-2 protein is a 505 amino acid protein with a predicted molecular weight of 55 kD
Distribution: Abundant expression in the brain
Function: Transcriptional activator
Interaction: SMAD3, SMAD4, UTF1, NBN, MRE11, XPO1, KAT5, CUL3
Biology Area: Cell Biology, DNA Repair/Replication, Transcription Factors
Antigen References: Abdel-Hafiz, H.A. et al. 1992. Mol Endocrinol. 6:2079-89. Gupta S, et al. 1995. Science. 267:389-93. Van Dam H, et al. 1995. EMBO J. 14:1798-811. Livingstone C, et al. 1995. EMBO J. 14:1785-97. Yamasaki T, et al. 2009. J Cell Biochem. 284:8567-81. Leslie MC, et al. 2009. J Cell Biochem. 94:25–38. Papassava P, et al. 2004. Cancer Res. 64:8573–84. Vlahopoulos SA, et al. 2008. BioEssays. 30:314–27. Bhoumik A, et al. 2005. Mol.Cell. 18:577–87. Li S, et al. 2010. Genes Cancer. 1:316–330.
Gene ID: 1386
UniProt: View information about ATF-2 on UniProt.org
Clone: W21106A
Regulatory Status: RUO
Other Names: activating transcription factor 2 formerly, ATF2, cAMP-responsive element-binding protein 2, CREB2, CREBP1, CRE-BP1, cyclic AMP-dependent transcription factor ATF-2, HB16, MGC111558, TREB7
Isotype: Rat IgG2b, κ