Biolegend, 631152, Purified anti-MAP2 Antibody, 100μg

Microtubules are 25 nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 undergoes alternative splicing which results in four isoforms: MAP2A, MAP2B, MAP2C and MAP2D. MAP2A and MAP2B result in proteins with an apparent molecular weight of ~280 kD. MAP2C and MAP2D are smaller molecular weight proteins which correspond protein bands running at ~70-75 kD on SDS-PAGE gels. All these MAP2 isoforms share a C-terminal core sequence which includes either three or four microtubule binding domains, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers for neuronal cells, their perikarya and neuronal dendrites.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of the N-terminus of human MAP2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone 1D7-B9-C8-F6) works with both Sodium Citrate H.I.E.R. (Cat. No. 928502) and Tris-EDTA pH 9.0 (10mM Tris, 1mM EDTA) antigen retrieval buffers, however, signal to noise ratio is higher using Sodium Citrate. This antibody (clone 1D7-B9-C8-F6) is not suitable for immunocytochemistry.
RRID: AB_3097544 (BioLegend Cat. No. 631151) AB_3097544 (BioLegend Cat. No. 631152)
Structure: MAP2 is a 1,827 amino acid protein with a predicted molecular weight of 199.5 kD.
Distribution: Cellular distribution: plasma membrane, cytoskeleton, nucleus, and cytosol Tissue distribution: Predominantly in the nervous system
Function: Microtubule assembly
Interaction: MAP2 interacts with microtubules. It has also been reported to interact with KNDC1 via KIND2.
Cell Type: Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Microtubules
Antigen References: De Camilli P, et al. 1984. Neuroscience. 11:817-46. Bloom GS, et al. 1984. J Cell Biol. 98:320-30. Maschietto M, et al. 2013. Front Cell Neurosci. 25:7. La Via L,et al. 2013. Nucelic Acids Res. 41:617-31. Ji B, et al. 2014. Hum Mol Genet. 23:5683-705. Fu X, et al. 2013. J Neurosci. 33:709.
Gene ID: 4133
UniProt: View information about MAP2 on UniProt.org
Clone: 1D7-B9-C8-F6
Regulatory Status: RUO
Other Names: Microtubule-associated protein 2 (MAP2)
Isotype: Mouse IgG2b, κ