Biolegend, 632853, Ultra-LEAF™ Purified anti-mouse HVEM Recombinant Antibody, 100μg

HVEM (Herpes virus entry mediator), also known as TNFRSF14, is a member of the tumor necrosis factor (TNF) receptor family that mediates herpes virus entry into cells during infection. Upon overexpression, HVEM activates NF-κB and AP-1 through a TNF receptor-associated factor (TRAF)-mediated mechanism. HVEM ligands belong to two distinct families: the TNF-related cytokines LIGHT and lymphotoxin-α, and the Ig-related membrane proteins BTLA and CD160. HVEM and its ligands have been involved in the pathogenesis of various autoimmune and inflammatory diseases, tumor progression and resistance to immune response. It has been demonstrated that HVEM induces a novel signaling pathway downstream leading to signal transduction and activation of STAT3 in epithelial cells. Since STAT3 regulates the expression of genes important for host defense in epithelial cells, as well as the differentiation of retinoid-related orphan receptor RORγt+ driven Th17, and innate lymphoid cells. This finding suggests that HVEM may play an important role in mucosal host defense.
100μg
Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Mouse HVEM-Fc Chimera
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.1 µg/mL recombinant mouse HVEM-Fc chimera to 1 µg/mL immobilized recombinant mouse BTLA-Fc. ND50 range: 0.1 – 0.6 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3097583 (BioLegend Cat. No. 632853) AB_3097583 (BioLegend Cat. No. 632854)
Structure: Dimer
Distribution: Spleen, thymus, lung, macrophages, B cells and T cells
Function: HVEM plays a role in mucosal host defense, autoimmune and inflammatory diseases, and tumor progression.
Ligand/Receptor: BTLA, CD160, LIGHT, lymphotoxin-α
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References: Pasero C, et al. 2012. Curr Opin Pharmacol. 12:478-85. Hsu H, et al. 1997. J Biol Chem. 272:13471-4. Mauri DN, et al. 1998. Immunity. 8:21-30. Montgomery RI, et al. 1996. Cell. 87:427-36. Shui JW, et al. 2012. Nature. 488:222-5.
Gene ID: 230979
UniProt: View information about HVEM on UniProt.org
Clone: QA19A74
Regulatory Status: RUO
Other Names: Herpesvirus Entry Mediator, HVEA, TR2, Tumor Necrosis Factor Receptor Superfamily 14, TNFRSF14
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.