Biolegend, 633351, Purified anti-HIF1A Antibody, 25μg

HIF1, hypoxia induced factor 1, is a heterodimeric basic helix-loop-helix transcription factor composed of HIF1α and HIF1β. Under normoxic conditions, HIF1α undergoes prolyl hydroxylation which leads to degradation of HIF1α by the proteasome. Oxygen-starvation inhibits prolyl hydroxylation and prevents proteasomal degradation of HIF1α, leading to stabilization of HIF1. HIF1α contains a nuclear localization sequence, and following nuclear translocation HIF1α binds to the core DNA sequence 5'-[AG]CGTG-3' within the hypoxia responsive element (HRE). HIF1 is a master regulator of the response to hypoxia and induces transcription of multiple genes including VEGF, glucose transporters, and proteins that aid in adaptation to hypoxic conditions. HIF1 protein is also involved in the regulation of angiogenesis, erythropoiesis, and energy metabolism.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Fragment of human HIF1A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality testedWB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.125 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone W22058A) is not recommended for immunocytochemistry (ICC). For IHC-P, antigen retrieval using Tris-EDTA pH 9.0 buffer (10mM Tris, 1mM EDTA) is recommended. Antigen retrieval with Sodium Citrate H.I.E.R. (Cat. No. 928502) is not recommended due to low signal.
RRID: AB_3097615 (BioLegend Cat. No. 633351) AB_3097615 (BioLegend Cat. No. 633352)
Structure: HIF1A is an 826 amino acid protein with a predicted molecular weight of 92.7 kD.
Distribution: Cytoplasmic in normoxia, nuclear in response to hypoxia
Function: Transcription factor, oxygen sensor
Interaction: Forms a dimer with Hif1β. Prolylation conducted by prolyl hydroxylase (PHD) and HIF prolyl hydroxylase (HPH), and subsequent degradation is mediated by von Hippel-Lindau tumor suppressor (VHL).
Antigen References: Wang GL, et al. 1995. Proc Natl Acad Sci USA. 92:5510-4. Lee C, et al. 2020. Biomed J. 43:434-437. Rashid M, et al. 2021. Gene. 798:145796.
Gene ID: 3091
UniProt: View information about HIF1A on UniProt.org
Clone: W22058A
Regulatory Status: RUO
Other Names: hypoxia inducible factor 1 alpha (Hif1α), ARNT-interacting protein, basic-helix-loop-helix-PAS protein MOP1
Isotype: Rat IgG2a, κ