Biolegend, 634052, Purified anti-Cyclin E1 Antibody, 100μg

Cyclin E1 is a crucial regulator of cell cycle progression. Cyclins are proteins that play a pivotal role in the control of cell division by interacting with cyclin-dependent kinases (CDKs). Cyclin E1 forms a complex with CDK2 to phosphorylate target proteins and facilitate the initiation of DNA synthesis. Cyclin E1 specifically governs the G1-to-S phase transition, accumulating during G1 and then being degraded as the cell proceeds through S phase. This regulatory function ensures proper cell cycle progression and is essential for maintaining genomic stability. Aberrations in Cyclin E1 expression or activity have been associated with various cancers, emphasizing its significance in normal cellular processes and highlighting its potential as a therapeutic target in the context of cancer treatment.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human Cyclin E1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, the suggested use of this reagent is 1.25 - 10.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunohistochemistry (IHC), we recommend antigen retrieval with either Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) or Sodium Citrate (Cat. No. 928502). For immunocytochemistry (ICC), we recommend fixation/permeabilization with either 100% ice-cold methanol, or fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X or 100% ice-cold methanol. This antibody (clone W20169G) is not suitable for Western blot (WB) or intracellular flow cytometry (ICFC).
RRID: AB_3106160 (BioLegend Cat. No. 634051) AB_3106160 (BioLegend Cat. No. 634052)
Structure: Cyclin E1 is a 410 amino acid protein with a predicted molecular weight of 47.1 kD.
Distribution: Nucleus
Function: Cell cycle regulation
Interaction: CDK2, UHRF2
Biology Area: Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication
Antigen References: Fagundes R and Teixeira LK. 2021. Front Cell Dev Biol. 9:774845. Lew DJ, et al. 1991. Cell. 66:1197-206. Zeng J, et al. 2023. Cell. 186:528-542. Chu C, et al. 2021. Trends Cell Biol. 31:732-746.
Gene ID: 898
UniProt: View information about Cyclin E1 on UniProt.org
Clone: W20169G
Regulatory Status: RUO
Other Names: G1/S-specific cyclin-E1 (CCNE1), CCNE, pcyclin E1
Isotype: Rat IgG2a, κ