Biolegend, 634101, Purified anti-Caspase-3 Antibody, 125microl

Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8 , and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin. The poly6341 antibody has been shown to be useful for western blotting, immunoprecipitation and immunohistochemistry of human Human caspase-3 precursor.
125microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Peptide mapping to the amino terminal domain of human caspase-3 prescursor
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. DO NOT FREEZE.
Application: WB - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 25µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product may contain other non-IgG subtypes.
Additional Product Notes: The 125 µL size can be used for approximately 5 Western blots.
Product Citations: Linetsky M, et al. 2020. Free Radic Biol Med. 152:280. PubMed Ramón-Luing LA, et al. 2022. Pathogens. 11:. PubMed
RRID: AB_2069874 (BioLegend Cat. No. 634101)
Structure: Peptidase family C14, heterotetramer. Two isoforms, pro-Casp3 32 kD
Interaction: Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo
Bioactivity: Involved in caspase activation cascade responsible for apoptosis execution. Cleaves/activates SREBPs, Casp6, Casp7, Casp9
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References: 1. Goldberg Y, et al. 1996. Nat. Genet. 13:442. 2. Fernandes-Alnemri T, et al. 1996. P. Natl. Acad. Sci. USA 93:7464. 3. Slee E, et al. 2001. J. Biol. Chem. 276:7320. 4. Woo M, et al. 2003. Nat. Immunol. 4:1016.
Regulation: Inhibited by dominant negative isoform, c-IAP1, c-IAP2, XIAP, survivin, Livin. Activated when cleaved by Casp6, -8, -10, granzyme B, Apaf-1 into large p17 and small p12 subunits
Gene ID: 12367
UniProt: View information about Caspase-3 on UniProt.org
Clone: Poly6341
Regulatory Status: RUO
Other Names: Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1
Isotype: Rabbit Polyclonal IgG