Biolegend, 634451, Purified anti-PP2A alpha Antibody, 25μg

PP2A alpha (also known as Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform or PPP2CA) is the catalytic subunit of protein phosphatase 2A (PP2A), which is involved in a wide range of cellular processes through its ability to dephosphorylate serine and threonine residues on target proteins. PPP2CA modulates the activity of proteins involved in cell cycle progression, such as cyclin-dependent kinases (CDKs), and is essential for the proper transition between different phases of the cell cycle, particularly G1/S and G2/M checkpoints. It also plays a critical role in DNA damage response, ensuring repair mechanisms are activated. Beyond cell cycle regulation, PP2A alpha influences various signaling cascades, including the MAPK, AKT, and WNT pathways, impacting cell proliferation, survival, and differentiation. Furthermore, PP2A alpha’s function in dephosphorylating tau proteins is significant in the context of neurodegenerative diseases, such as Alzheimer's disease, where abnormal phosphorylation of tau is a hallmark feature.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant full-length human PP2A alpha
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). In human kidney tissue, this antibody is recommended for use at 10 µg/mL, as non-specific staining in tubules may be observed at lower concentrations. This product is not recommended for use in immunocytochemistry (ICC), intracellular flow cytometry (ICFC), or Western blot (WB).
RRID: AB_3683480 (BioLegend Cat. No. 634451) AB_3683480 (BioLegend Cat. No. 634452)
Structure: PP2A alpha is a 309 amino acid protein with a predicted molecular weight of 35.6 kD. Catalytic subunit of protein phosphatase 2A (PP2A).
Distribution: Cytoplasmic and nuclear
Function: Serine/threonine phosphatase which regulates enzymes and signal transduction pathways.
Interaction: PP2A alpha interacts with a wide variety of proteins including AKT, c-Myc, BCL2, cyclin-dependent kinases, MAPK, CD56 and Tau
Cell Type: Neurons
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Ronk H, et al. 2022. Cancer Biol Med. 19:1428-39. Reynhout S and Janssens V. 2019. Biochim Biophys Acta Mol Cell Res. 1866:31-50. Lei WL, et al. 2021. Front Cell Dev Biol. 9:638559. Wlodarchak N and Xing Y. 2016. Crit Rev Biochem Mol Biol. 52:162-84.
Gene ID: 5515
UniProt: View information about PPSA alpha on UniProt.org
Clone: W18203A
Regulatory Status: RUO
Other Names: PPP2CA, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha (PP2AA), Replication protein C (RP-C)
Isotype: Rat IgG2a, κ