Biolegend, 634502, Purified anti-Alix Antibody, 100μg

Alix (ALG-2-interacting protein X) is an adaptor protein that was first described for its capacity to bind to the calcium-binding protein ALG-2, the expression of which seemed necessary for cell death. The predicted molecular weight is approximately 96 kD. Alix contains an N-terminal Bro1 domain and a C-terminal proline-rich domain (PRD). The PRD contains multiple polyproline motifs, which are potential docking sites for proteins containing SH3 domains, interact with multiple cellular Alix-binding partners that are involved in apoptotic induction, endosomal sorting, and endocytosis. The Bro1 domain interacts with CHMP4b, which is also involved in endosomal sorting.
100μg
Verified Reactivity: Human, Mouse
Reported Reactivity: Rat, Frog
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full-length human Alix protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 3A9 has been shown to be useful for Western blotting1, immunoprecipitation, and immunofluorescence2 staining of the human and mouse Alix protein. Clone 3A9 shows strong specificity for mouse Alix.
Application References(PubMed link indicates BioLegend citation): Morel L, et al. 2013. J Biol Chem. 288:7105. (WB) PubMed Pan S, et al. 2008. EMBO J. 27:2077. (IF) Ertunc ME, et al. 2015. J Lipid Res. 56:423. PubMed
Product Citations: Yang C, et al. 2011. PLoS One. 6:e22517. PubMed Stempels FC, et al. 2023. J Cell Biol. 222:. PubMed Inouye KE, et al. 2023. JCI Insight. . PubMed Yim WW, et al. 2022. FEBS Lett. 596:991. PubMed Dong H, et al. 2022. Proc Natl Acad Sci U S A. 119:e2123247119. PubMed Tan JX, et al. 2022. Nature. 609:815. PubMed Jia X, et al. 2022. J Cell Biol. 221: . PubMed Burbidge K, et al. 2022. Autophagy. 18:1020. PubMed Jia J, et al. 2020. Dev Cell. 69:52. PubMed Suthar J, et al. 2020. Anal Chem. 92:4082. PubMed Morel L, et al. 2013. J Biol Chem. 288:7105. PubMed Yoon S, et al. 2017. Immunity. 47:51:00. PubMed Gulluni F, et al. 2021. Science. 374:eabk0410. PubMed Gorji-Bahri G, et al. 2021. Cell Biol Int. 45:1098. PubMed Valkov N, et al. 2021. Life Sci Alliance. 4:. PubMed Hüttenhain R et al. 2019. Cell Host Microbe. 26(1):86-99 . PubMed Moore D, et al. 2019. Stem Cells Int. 2019:8710180. PubMed Baba T, et al. 2021. Cell Death Dis. 12:322. PubMed Davis OB, et al. 2020. Developmental Cell. 56(3):260-276.e7. PubMed Gärtner K, et al. 2018. Cancer Med. 11:39. PubMed Ko J, et al. 2018. Lab Chip. 3.261805556. PubMed Skowyra ML, et al. 2018. Science. 360:6384. PubMed Gorji-Bahri G, et al. 2021. J Cell Biochem. 122:425. PubMed Andre-Gregoire G, et al. 2022. iScience. 25:105118. PubMed Gunasekaran M, et al. 2020. J Heart Lung Transplant. 39:379. PubMed Hiraga C, et al. 2021. Sci Rep. 11:3326. PubMed Chand S, et al. 2021. J Extracell Vesicles. 10:e12177. PubMed Ertunc M, et al. 2015. J Lipid Res. 56:423. PubMed Claude-Taupin A, et al. 2021. Nat Cell Biol. 23:846. PubMed Millarte V, et al. 2022. EMBO Rep. 23:e53429. PubMed Jia J, et al. 2020. Mol Cell. 951:77. PubMed Bæk R, et al. 2016. J Immunol Methods. 438:11-20. PubMed Gunasekaran M, et al. 2018. J Immunol. 200:2535. PubMed Schiller LT, et al. 2018. Mol Ther Methods Clin Dev. 15:1630. PubMed
RRID: AB_2268110 (BioLegend Cat. No. 634501) AB_2162471 (BioLegend Cat. No. 634502)
Structure: Belongs to a conserved family of proteins that have in common an N-terminal Bro1 domain and a C-terminal proline-rich domain. Predicted molecular weight approximately 96 kD.
Distribution: Cytoplasm. Alix is also abundant within extracellular vesicles known as exosomes, and enriched in virions that are released from cells under the direction of the HIV-1 Gag protein.
Function: Adaptor in signal transduction pathways. Involved in apoptosis and endosomal membrane trafficking and viral budding.
Interaction: ALG2, SH3GL2, H-Vps28, CHMP4B, CHMP4A, CHMP4C, SETA, CIN85, Src
Biology Area: Cell Biology
Antigen References: 1. Wu Y, et al. 2000. Differentiation 67:139. 2. Missotten M, et al. 1999. Cell Death Differ. 6:124. 3. Odorizzi G. 2006. J Cell Sci. 119:3025.
Gene ID: 10015
UniProt: View information about Alix on UniProt.org
Clone: 3A9
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: PDCD6IP, PDCD6 interacting protein, AIP1, Hp95
Isotype: Mouse IgG1, κ