Biolegend, 636054, Ultra-LEAF™ Purified anti-human Cripto (TDGF-1) Antibody, 1mg

Cripto is a glycosylphosphatidylinositol (GPI)-anchored Type I cell membrane protein that belongs to the epidermal growth factor (EGF)-Cripto-1-FRL-1-Cryptic (CFC) family of proteins (EGF-CFC). In addition to Cripto, another three members of the EGF-CRC family have been described: Criptic, Xenopus FRL-1, and zebrafish one-eyed pinhead (0EP). Cripto contains a signal sequence, an EGF-like domain, a cysteine-rich region motif (CFC domain), and a hydrophobic COOH-terminus. Cripto plays an important role in maintaining embryonic stem cell pluripotency and tumorigenesis. Cripto acts as a co-receptor for Nodal, a TGFb family member that plays a crucial role in mesoderm and endoderm formation. Nodal and Cripto form complex with type I Activin receptors, Alk4/7 (ACVR1B/ACVR1C), and type II Activin receptors, ActRIIA/ActRIIB (ACVR2A/ACVR2B). Cripto is also known to bind Furin and Pace4 that process Nodal proteolytic maturation and supports Nodal signaling. A soluble form of Cripto is secreted by tumor cells and it lacks the GPI anchor region which is removed by GPI-phospholipase D. The soluble Cripto promotes endothelial cell migration and possibly tumor angiogenesis. More recent data showed that a GPI phospholipase A2 called post-glycosylphosphatidylinositol attachment to proteins 6 (PGAP6) in involved in the in the shedding of Cripto. PGAP6 produces Cripto bearing lyso-GPI which is released from the membrane, and subsequently Cripto is released from the lyso–GPI by GPI-phospholipase D.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Human Cripto
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 450 ng/mL Recombinant Human Cripto (carrier-free) to 1 μg/mL immobilized Recombinant Human Activin R1B/ALK-4-Fc Chimera (carrier-free). ND50 range: 0.8-5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3675173 (BioLegend Cat. No. 636053) AB_3675173 (BioLegend Cat. No. 636054)
Distribution: Mainly expressed during embryogenesis, embryonic stem cells, induced in pluripotent stem cells. Low expression in adult tissues, hematopoetic stem cells, mammary epithelial cells, adipose tissue, pancreas, and endometrium.
Function: Induces cell growth, migration, invasion, and epithelial to mesenchymal transition. Participates in tumorigenesis, cancer cell proliferation and survival.
Interaction: Interacts with Nodal, ALK4, Furin, PACE4, and GRP78
Ligand/Receptor: Co-receptor of Nodal, GDF1, and GDF3
Biology Area: Cancer Biomarkers, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Ciccodicola A, et al. 1989. EMBO J. 8:1987-91. Beck S, et al. 2002. Nature Cell Biology. 4:981-5. Gray PC, et al. 2003. Proc Natl Acad Sci USA. 100:5193-8. Xing PX, et al. 2004. Cancer Res. 64:4018-23. Watanabe K, et al. 2007. J Biol Chem. 282:35772-86. Blanchet MH, et al. 2008. EMBO J. 27:2580-91. Gray PC and Vale W. 2012. FEBs Letter. 586:1836-45. Lee GH, et al. 2016. J Cell Biol. 215:705-718. Zoni E, et al. 2017. Oncogene. 36:4739-4749.
Gene ID: 6997
UniProt: View information about Cripto on UniProt.org
Clone: W22247A
Regulatory Status: RUO
Other Names: Teratoma-derived growth factor 1 (TDGF-1), Epidermal Growth Factor-Like Cripto Protein (CR1), Cripto-1 Growth Factor, CR, CRGF, CR-1
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.