Biolegend, 641251, Purified anti-HMGB1 Antibody, 25μg

High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains. Human HMGB1 is 100% identical to the sequence of canine HMGB1, and 99% identical to mouse, rat, bovine and porcine HMGB1. HMGB1 is a widely expressed and highly abundant protein. It was originally discovered as a nuclear protein but is now known to also be secreted and play a significant role in extracellular signaling as a proinflammatory cytokine. It acts as an inflammatory mediator that promotes monocyte migration, cytokine secretion, and mediate T cell-dendritic cell interaction. In addition, activated monocytes, macrophages, and dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils.  The cytokine activity of HMGB1 is restricted to the HMG B box, while the A box is associated with the helix-loop-helix domain of transcription factors. Although HMGB1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant mouse HMGB1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use of this antibody in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol. For use of this antibody in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with either of the following: -True-Phos™ Perm Buffer (Cat. No. 425401). -True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). *Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) is not recommended with this antibody.
RRID: AB_3716968 (BioLegend Cat. No. 641251) AB_3716968 (BioLegend Cat. No. 641252)
Structure: 215 amino acids with predicted molecular weight of 25 kD
Distribution: Nucleus
Interaction: CXCL12, RAG Complex, BECN1, KPNA1
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Thomas JO and Travers AA. 2001. Trends Biochem Sci. 26:167. Müller S, et al. 2004. J Intern Med. 255:332. Campana L, et al. 2008. Curr Opin Immunol. 20:518. Klune JR, et al. 2008. Mol Med. 14:476. Dumitriu IE, et al. 2005. Trends Immunol. 26:381. Bonaldi T, et al. 2003. EMBO J. 22:5551.
Gene ID: 3146
UniProt: View information about HMGB1 on UniProt.org
Clone: A15164F
Regulatory Status: RUO
Other Names: High mobility group protein B1, High mobility group protein 1, HMG-1,
Isotype: Mouse IgG2a, κ