Biolegend, 642151, Purified anti-CUL3 Antibody, 25μg

CUL3 (Cullin-3) is a key component of the ubiquitin-proteasome system (UPS), an essential pathway for regulating protein degradation in human cells. It functions as part of an E3 ubiquitin ligase complex, which tags specific proteins with ubiquitin molecules, marking them for degradation by the proteasome. CUL3 is a member of the Cullin family of proteins, which provide a structural scaffold for the assembly of multi-subunit ubiquitin ligases, termed Cullin-RING ligases (CRLs). These ligases play a central role in maintaining protein homeostasis by controlling the turnover of numerous regulatory proteins involved in critical cellular processes such as cell cycle progression, signal transduction, transcription, and stress responses. CUL3 forms a complex with a RING-domain protein, RBX1, which recruits the E2 ubiquitin-conjugating enzyme, and various substrate adaptors known as BTB domain-containing proteins. The BTB proteins determine substrate specificity, allowing CUL3 to target a wide range of proteins for degradation. This ability to selectively degrade proteins makes CUL3 a crucial regulator of several physiological and pathological processes.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human CUL3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone W21054D), is not recommended for use in either immunocytochemistry (ICC) or immunohistochemistry (IHC-P).
RRID: AB_3675176 (BioLegend Cat. No. 642151) AB_3675176 (BioLegend Cat. No. 642152)
Structure: CUL3 is a 768 amino acid length protein with a predicted molecular weight of 88.9 kD.
Distribution: Located in nucleus, Golgi apparatus, and cytoplasm
Function: Mediates the ubiquitination and subsequent proteasomal degradation of target proteins
Interaction: A component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes
Biology Area: Cell Proliferation and Viability, Ubiquitin/Protein Degradation
Molecular Family: Adaptor Proteins, Enzymes and Regulators
Antigen References: Canning P, et al, 2013, J Biol Chem.288:7803-7814. McGourty C, et al. 2016. Cell. 167:525-538.e14. Dong Z, et al. 2019. Neuron. 105:475-490.e6.
Gene ID: 8452
UniProt: View information about CUL3 on UniProt.org
Clone: W21054D
Regulatory Status: RUO
Other Names: Cullin-3, CUL-3, Cullin3, PHA2E, KIAA0617
Isotype: Rat IgG2b, λ