Biolegend, 643802, Purified anti-β-actin Antibody, 100μg

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.
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RRID: AB_2223201 (BioLegend Cat. No. 643801) AB_2223199 (BioLegend Cat. No. 643802)
Structure: Actin, cytoplasmic 1
Distribution: Ubiquitously expressed in the cytoplasm of all eukaryotic cells
Function: Actins are highly conserved proteins that are involved in cell motility
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microfilaments
Antigen References: 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
Gene ID: 11461
UniProt: View information about beta-actin on UniProt.org
Clone: 2F1-1
Regulatory Status: RUO
Other Names: Actin, cytoplasmic 1
Isotype: Mouse IgG2b, κ