Biolegend, 643806, Brilliant Violet 421™ anti-β-actin Antibody, 100μg

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.
Application References(PubMed link indicates BioLegend citation): Lawson BR, et al. 2007. J. Immunol. 178:5366. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053. Yanagiya T,et al.2007.Obesity.15:572. KishidaT,et al. 2007.J. Immunol. 179:8554. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. Toltlt LJ,et al. 2008.J. Immunol. 181:2165. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. Ikeda D, et al. 2008. Endocrinology. 149:6037. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
RRID: AB_2566300 (BioLegend Cat. No. 643806)
Structure: Actin, cytoplasmic 1
Distribution: Ubiquitously expressed in the cytoplasm of all eukaryotic cells
Function: Actins are highly conserved proteins that are involved in cell motility
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microfilaments
Antigen References: 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
Gene ID: 11461
UniProt: View information about beta-actin on UniProt.org
Clone: 2F1-1
Regulatory Status: RUO
Other Names: Actin, cytoplasmic 1
Isotype: Mouse IgG2b, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.