Biolegend, 643901, Purified anti-Cdk2 Antibody, 25μg

Cyclin-dependent kinase 2 (Cdk2), also known as cell division protein kinase 2, is an enzyme involved in cell cycle control. Binding of Cdk2 to Cyclin E in G1 phase is required for G1 to S phase transition, while binding of Cdk2 to Cyclin A is required to progress through the S phase. Clone AN4.3 does not recognize other CDKs.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CDK2 recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Shi L, et al. 2014. J. Immunol. 192:649. PubMed
Product Citations: Cui M, et al. 2024. Oncogene. 2708:43. PubMed Shi L, et al. 2014. J Immunol. 192:649. PubMed
RRID: AB_2276103 (BioLegend Cat. No. 643901)
Structure: There are two isoforms reported in human with predicted MW of 30 kD, 34 kD, respectively; there are also two isoforms reported in mouse with predicted MW of 34 kD, 39 kD, respectively.
Distribution: Nuclear protein, predicted molecular weight 34 kD.
Function: Involved in the control of the cell cycle
Interaction: Cyclins A, B1, B3, D, or E.
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: 1. Jeffrey PD, et al. 1995. Nature 376:313
Gene ID: 1017
UniProt: View information about Cdk2 on UniProt.org
Clone: AN4.3
Regulatory Status: RUO
Other Names: p33(CDK2)
Isotype: Mouse IgG2a, κ