Biolegend, 644710, Brilliant Violet 421™ anti-GFAP Antibody, 100μg

GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Bovine spinal cord homogenate
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-F - Quality testedICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.25 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221. Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
Product Citations: Hill JD, et al. 2019. Brain Behav Immun. 76:165. PubMed Osaki T, et al. 2020. Nat Protoc. 15:421. PubMed Fan KQ, et al. 2020. Cell. 179(4):864-879.e19.. PubMed Sanmarco LM, et al. 2021. Nature. 590:473. PubMed Rizzo MD, et al. 2019. J Neuroimmunol. 333:576969. PubMed
RRID: AB_2566685 (BioLegend Cat. No. 644710)
Structure: Around 50 kD
Distribution: Only expressed in astrocytes in the central nervous system.
Function: Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
Cell Type: Astrocytes
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Gene ID: 2670
UniProt: View information about GFAP on UniProt.org
Clone: 2E1.E9
Regulatory Status: RUO
Other Names: Glial fibrillary acid protein
Isotype: Mouse IgG2b
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.