Biolegend, 644806, PerCP/Cyanine5.5 anti-T-bet Antibody, 100μg

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Application References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations: Read KA, et al. 2023. Nat Commun. 14:1652. PubMed Nunes-Santos CJ, et al. 2023. Nat Commun. 14:3708. PubMed Wang W, et al. 2022. Cell Rep. 41:111582. PubMed Glaubitz J, et al. 2023. Gut. :. PubMed Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed Zander R, et al. 2022. Immunity. 55:475. PubMed Frasca D, et al. 2019. PLoS One. 14:e0219545. PubMed Amelio P, et al. 2017. PLoS Negl Trop Dis. 10.1371/journal.pntd.0005817. PubMed Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed Huanosta-Murillo E, et al. 2021. Front Immunol. 12:668369. PubMed Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Marro BS, et al. 2019. Cell Rep. 29:3293. PubMed Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed Webb ER, et al. 2022. iScience. 25:104995. PubMed Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed Hurton L, et al. 2016. Proc Natl Acad Sci U S A. 113: E7788 - E7797. PubMed Nabatanzi R, et al. 2021. BMC Immunol. 22:59. PubMed Minns D, et al. 2021. Nat Commun. 12:1285. PubMed Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
RRID: AB_1595593 (BioLegend Cat. No. 644805) AB_1595488 (BioLegend Cat. No. 644806)
Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009
UniProt: View information about T-bet on UniProt.org
Clone: 4B10
Regulatory Status: RUO
Other Names: T-box expressed in T cells, T box 21, TBLYM
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.