Biolegend, 646003, PE anti-Syk Antibody, 25μg

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Syk peptide aa306-333
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Application References(PubMed link indicates BioLegend citation): Chu D, et al. 1999. J. Immunol. 163:2610. Ackermann JA, et al. 2015. J Immunol. 194:4650. PubMed
Product Citations: Leonardi I, et al. 2022. Cell. 185:831. PubMed Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed Li X, et al. 2018. Cell Host Microbe. 24:847. PubMed
RRID: AB_2565305 (BioLegend Cat. No. 646003) AB_2565306 (BioLegend Cat. No. 646004)
Structure: Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Distribution: Cytoplasmic protein, widely expressed in hematopoietic cells
Function: Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790. 2. Toyabe SL, et al. 2001.Immunology 103:164.
Gene ID: 6850
UniProt: View information about Syk on UniProt.org
Clone: 5F5
Regulatory Status: RUO
Other Names: Spleen tyrosine kinase, protein tyrosine kinase SYK
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.