Biolegend, 646151, Purified anti-Pan AKT Antibody, 25μg

The AKT family of proteins, also known as Protein Kinase B (PKB), consists of three isoforms: AKT1, AKT2, and AKT3. These serine/threonine kinases are pivotal in regulating various cellular processes, including glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. AKT1 is primarily involved in cellular survival pathways and is a key player in inhibiting apoptosis, promoting cell growth, and protein synthesis. AKT2 is crucial in the insulin signaling pathway, facilitating glucose transport and maintaining glucose homeostasis. AKT3 is predominantly expressed in the brain and is essential for brain development. Dysregulation of AKT signaling is associated with numerous diseases, including cancer, diabetes, and neurological disorders such as Alzheimer's disease. Overexpression or mutations in AKT genes can lead to uncontrolled cell growth and survival, contributing to tumorigenesis in various cancers. Additionally, aberrant AKT signaling is implicated in insulin resistance and type 2 diabetes, as well as neurodegenerative diseases.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide for human AKT
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 2.5 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody detects all 3 isoforms of AKT protein kinases (AKT1, AKT2, AKT3).
Additional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using either True-Phos™ Perm Buffer (Cat. No. 425401), or Cyto-Fast Fix/Perm Buffer set (Cat. No. 426803) For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using either PFA-Triton X-100, or Methanol only. For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).
RRID: AB_3699072 (BioLegend Cat. No. 646151) AB_3699072 (BioLegend Cat. No. 646152)
Structure: AKT Molecular Weight: ~ 56 kDa
Distribution: Nucleoplasm and cytoplasm
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immuno-Oncology
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Krzeslak A, et al. 2011. Endocrine Pathology. 22:206. Jian K, et al. 2003. Blood. 101:236. Manning BD, et al. 2017. Cell. 169:381. Song M, et al. 2019. Cancer Res. 79:1019. Revathidevi S, et al. 2019. Semin Cancer Biol. 59:80.
Gene ID: 2072081000
UniProt: View information about AKT on UniProt.org
Clone: A21001A
Regulatory Status: RUO
Other Names: Protein kinase B, PKB, RAC-Alpha, RAC-alpha serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-gamma serine/threonine-protein kinase
Isotype: Mouse IgG2b, κ