Biolegend, 647501, Purified anti-XBP-1s Antibody, 25μg

XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of human HLA DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 upregulates as part of the ER stress response, also known as the unfolded protein response. Unspliced XBP-1 is 261 amino acids and migrates on the SDS-PAGE around 33 kD; spliced XBP-1, recognized by clone 143F, is 371 amino acids and migrates around 55 kD.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: XBP-1s recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ChIP - Verified ICC, IHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 to 2.0 µg per mL. For ChIP application, use 2 µg of antibody per 10 µg of chromatin per IP. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence14 and immunohistochemical staining of PFA-fixed paraffin-embedded sections1,14.
Additional Product Notes: Using this antibody at a ratio higher than 1:5 (µg antibody per µg of chromatin) will increase background.
Application References(PubMed link indicates BioLegend citation): Tsang KY, et al. 2007. PLOS Biol. 5:568. (IHC) PubMed Farhan H, et al. 2008. EMBO J. 27:2043 (WB) PubMed Farhan H, et al. 2010. J. Cell Biol. 189:997. PubMed Feng-Jin G, et al. 2010. Cell Signal. [Epub ahead of print] Wang L, et al. 2011. Hum Mol Genet. PubMed Li J, et al. 2011. J. Biol Chem. 286:4912. PubMed Wang L, et al. 2011. Hum Mol Genet. 20:1008. PubMed Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed Shirley CM, et al. 2011 Blood. 117:6297. PubMed Vidal RL., et al. 2012. Hum Mol Genet. PubMed Byrd AE, et al. 2012. J Cell Biol. 196:689. PubMed Dickie LJ, et al. 2012. Ann rheum Dis. 71:2035. PubMed Bonetti P, et al. 2013. Blood. 122:2233. PubMed Maestre L, et al. 2009. Haematologica. 94:419. (IF, IHC) Rodriguez M, et al. 2014. J Biol Chem. 289:22942. PubMed
Product Citations: El-Gazzar A, et al. 2023. EMBO Mol Med. 15:e16834. PubMed Pietrafesa G, et al. 2023. J Transl Med. 21:340. PubMed Hattori K, et al. 2021. Oncol Lett. 22:680. PubMed Zielke S, et al. 2020. Autophagy. 1:. PubMed Li J et al. 2018. Cell chemical biology. 25(11):1350-1358 . PubMed Dickie L, et al. 2012. Ann Rheum Dis. 71:2035. PubMed Wang L, et al. 2011. Hum Mol Genet. 1.533333333. PubMed Syx D, et al. 2021. PLoS Genet. 17:e1009339. PubMed Zang M, et al. 2020. Oncogenesis. 9:31. PubMed Cai X, et al. 2015. Exp Cell Res. Available online 29 August 2015. PubMed Byrd A, et al. 2012. J Cell Biol. 196:689. PubMed Miyoshi A, et al. 2019. FASEB J. 33:3575. PubMed Li X, et al. 2018. Sci Rep. 7:2013. PubMed Lindner P, et al. 2020. Cell Commun Signal. 18:12. PubMed Shirley C, et al. 2011. Blood. 117:6297. PubMed Rinaldi R, et al. 2021. Cells. 10:. PubMed Vidal R, et al. 2012. Hum Mol Genet. 2.434027778. PubMed Luhr M, et al. 2019. J Biol Chem. 294:8197. PubMed Farhan H, et al. 2010. J Cell Biol. 189:997. PubMed Li J, et al. 2011. J Biol Chem. 286:4912. PubMed Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed Talty A, et al. 2019. Cell Death Dis. 10:622. PubMed Walter F, et al. 2022. Front Cell Dev Biol. 10:915065. PubMed Chen W, et al. 2022. Nat Commun. 13:6796. PubMed Efimova I, et al. 2021. Front Immunol. 12:643144. PubMed Devireddy S, et al. 2022. J Cell Biol. 221:. PubMed
RRID: AB_2241744 (BioLegend Cat. No. 647501) AB_2241743 (BioLegend Cat. No. 647502)
Distribution: Ubiquitously expressed.
Function: Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
Biology Area: Cell Biology
Antigen References: 1. Liou HC, et al. 1990. Science 247:1581. 2. Ponath PD, et al. 1993. J. Biol. Chem.268:17074. 3. Takahashi S, et al. 2002. Prostate 50:154.
Gene ID: 7494
UniProt: View information about XBP-1s on UniProt.org
Clone: 143F
Regulatory Status: RUO
Other Names: X-box binding protein 1
Isotype: Mouse IgG2a, κ